Abstract In this study, arbutin, an antibacterial skin-whitening agent was identified and quantified in blueberry samples using a developed high-performance liquid chromatographic (HPLC) method. The best instrumental conditions were obtained by performing step-by-step optimization studies. The highest arbutin signal was observed at a wavelength of 220 nm with the ultraviolet detector. Arbutin was eluted through a C18 column by a mobile phase composed of 89:10:1 (v/v/v) water:methanol:0.10 M hydrochloric acid. The analytical figures of merit were calculated from a calibration plot developed across the concentration range from 0.010 to 250 mg/L under the optimum conditions with a coefficient of determination value of 0.9999. The limits of detection and quantification values were 2.0 and 6.6 µg/L, respectively. Spiked recovery measurements were used to ascertain the applicability of the analytical method in different blueberry samples, and the obtained recovery values ranged between 93 and 100%. The analyte was exposed to simulated gastric fluid and ultraviolet radiation to ascertain its stability under these conditions.

中文翻译：

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蓝莓中熊果苷的准确、灵敏反相高效液相色谱测定及其在模拟胃液和紫外线照射下的稳定性表征

摘要 在本研究中，使用开发的高效液相色谱 (HPLC) 方法对蓝莓样品中的熊果苷（一种抗菌美白剂）进行了鉴定和定量。最佳仪器条件是通过逐步优化研究获得的。使用紫外检测器在 220 nm 波长处观察到最高的熊果苷信号。熊果苷通过 C18 柱被流动相洗脱，流动相由 89:10:1 (v/v/v) 水:甲醇:0.10 M 盐酸组成。分析品质因数是根据在最佳条件下在 0.010 至 250 mg/L 的浓度范围内绘制的校准曲线计算得出的，测定系数值为 0.9999。检测限和定量值分别为 2.0 和 6.6 µg/L。加标回收率测量用于确定分析方法在不同蓝莓样品中的适用性，获得的回收率值介于 93% 和 100% 之间。将分析物暴露于模拟胃液和紫外线辐射下，以确定其在这些条件下的稳定性。