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Sodium pheophorbide a controls cherry tomato gray mold (Botrytis cinerea) by destroying fungal cell structure and enhancing disease resistance-related enzyme activities in fruit
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.pestbp.2020.104581 Jing-Yu Ji 1 , Jing Yang 1 , Bo-Wen Zhang 2 , Shu-Ren Wang 1 , Guo-Cai Zhang 1 , Lian-Nan Lin 3
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.pestbp.2020.104581 Jing-Yu Ji 1 , Jing Yang 1 , Bo-Wen Zhang 2 , Shu-Ren Wang 1 , Guo-Cai Zhang 1 , Lian-Nan Lin 3
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Sodium pheophorbide a (SPA) is a natural photosensitizer. The present study investigated the antifungal activity and mechanism of SPA against Botrytis cinerea in vitro and in vivo. Its inhibitory effect was studied on the spore germination and mycelial growth of B. cinerea. The effects of SPA on cell wall integrity, cell membrane permeability, and mycelial morphology of B. cinerea were also determined. Additionally, how SPA effected B. cinerea in vivo was evaluated using cherry tomato fruit. The results showed that SPA effectively inhibited the spore germination and mycelial growth of B. cinerea under light conditions (4000 lx). SPA significantly affected both cell wall integrity and cell membrane permeability (P < .05). In addition, SEM analysis suggested that B. cinerea treated with SPA (12.134 mg/mL) showed abnormal mycelial morphology, including atrophy, collapse, flattening, and mycelial wall dissolution. In vivo tests showed that SPA could increase the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) significantly (P < .05); however, SPA had no significant effect on phenylalanine ammonia lyase (PAL) activity. In short, SPA could destroy the fungal cell structure and enhance disease resistance-related enzyme activity in cherry tomatoes, thereby controlling cherry tomato gray mold.
中文翻译：
脱镁叶酸钠a通过破坏真菌细胞结构和增强果实抗病相关酶活性来控制樱桃番茄灰霉病（Botrytis cinerea）
脱镁叶酸钠 (SPA) 是一种天然光敏剂。本研究在体外和体内研究了 SPA 对灰葡萄孢的抗真菌活性和机制。研究了其对灰霉病孢子萌发和菌丝生长的抑制作用。还确定了 SPA 对 B. cinerea 细胞壁完整性、细胞膜通透性和菌丝形态的影响。此外，还使用樱桃番茄果实评估了 SPA 如何在体内影响 B. cinerea。结果表明，SPA在光照条件下（4000 lx）有效抑制了灰霉病菌的孢子萌发和菌丝生长。SPA 显着影响细胞壁完整性和细胞膜通透性 (P < .05)。此外，SEM 分析表明用 SPA (12.134 mg/mL) 处理的 B. cinerea 显示出异常的菌丝体形态，包括萎缩、塌陷、扁平化和菌丝壁溶解。体内试验表明，SPA可显着提高超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性（P < .05）；然而，SPA对苯丙氨酸解氨酶（PAL）活性没有显着影响。总之，SPA可以破坏樱桃番茄的真菌细胞结构，增强抗病相关酶的活性，从而控制樱桃番茄灰霉病。
更新日期：2020-06-01
中文翻译：
脱镁叶酸钠a通过破坏真菌细胞结构和增强果实抗病相关酶活性来控制樱桃番茄灰霉病（Botrytis cinerea）
脱镁叶酸钠 (SPA) 是一种天然光敏剂。本研究在体外和体内研究了 SPA 对灰葡萄孢的抗真菌活性和机制。研究了其对灰霉病孢子萌发和菌丝生长的抑制作用。还确定了 SPA 对 B. cinerea 细胞壁完整性、细胞膜通透性和菌丝形态的影响。此外，还使用樱桃番茄果实评估了 SPA 如何在体内影响 B. cinerea。结果表明，SPA在光照条件下（4000 lx）有效抑制了灰霉病菌的孢子萌发和菌丝生长。SPA 显着影响细胞壁完整性和细胞膜通透性 (P < .05)。此外，SEM 分析表明用 SPA (12.134 mg/mL) 处理的 B. cinerea 显示出异常的菌丝体形态，包括萎缩、塌陷、扁平化和菌丝壁溶解。体内试验表明，SPA可显着提高超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性（P < .05）；然而，SPA对苯丙氨酸解氨酶（PAL）活性没有显着影响。总之，SPA可以破坏樱桃番茄的真菌细胞结构，增强抗病相关酶的活性，从而控制樱桃番茄灰霉病。
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