Plant cell walls constitute the extracellular matrix surrounding plant cells and are composed mainly of polysaccharides. The chemical makeup of the primary plant cell wall, and specifically, the abundance, localization and arrangement of the constituting polysaccharides are intimately linked with growth, morphogenesis and differentiation in plant cells. Visualization of the cell wall components is, therefore, a crucial tool in plant cell developmental studies. In this technical update, we present protocols for fluorescence visualization of cellulose and pectin in selected plant tissues and illustrate examples of some of the available labels that hold promise for live imaging of plant cell wall expansion and morphogenesis.

中文翻译：

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初级植物细胞壁中纤维素和果胶的荧光可视化

植物细胞壁构成植物细胞周围的细胞外基质，主要由多糖组成。主要植物细胞壁的化学组成，特别是组成多糖的丰度、定位和排列，与植物细胞的生长、形态发生和分化密切相关。因此，细胞壁成分的可视化是植物细胞发育研究的重要工具。在本次技术更新中，我们介绍了选定植物组织中纤维素和果胶的荧光可视化方案，并举例说明了一些有希望对植物细胞壁扩张和形态发生进行实时成像的可用标签。