Key message

In this manuscript, we disclosed the influence of light on the accumulation of storage reserves in B. napus embryos.

1. 1.

   Light induced the gene expression in the developing embryos of B. napus.

2. 2.

   Light promoted the starch synthesis in chloroplasts of B. napus embryos.

3. 3.

   Light enhanced the metabolic activity of storage reserve synthesis in B. napus embryos.

Abstract

Light influences the accumulation of storage reserves in embryos, but the molecular mechanism was not fully understood. Here, we monitored the effects of light on reserve biosynthesis in Brassica napus by comparing embryos from siliques grown in normal light conditions to those that were shaded or masked (i.e., darkened completely). Masked embryos developed more slowly, weighed less, and contained fewer proteins and lipids than control embryos. They also had fewer and smaller oil bodies than control embryos and lacked chloroplasts, where starch grains are usually synthesized. The levels of most amino acids, carbohydrates, and fatty acids were higher in masked embryos than in control or shaded embryos, whereas the levels of these metabolites in the masked endosperms were lower than those in control and shaded endosperm. Transcriptome analysis indicated that genes involved in photosynthesis (42 genes), amino acid biosynthesis (51 genes), lipid metabolism (61 genes), and sugar transport (13 genes) were significantly repressed in masked embryos. Our results suggest that light contributes to reserve accumulation in embryos by inducing the expression of metabolic genes, thereby enhancing the biosynthesis of storage reserves.

中文翻译：

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光诱导基因表达以增强欧洲油菜胚胎中储存储备的合成。

关键信息

在这份手稿中，我们揭示了光对欧洲油菜胚胎储存储备积累的影响。

1. 1.

   光诱导欧洲油菜发育胚胎中的基因表达。

2. 2.

   光促进了欧洲油菜胚胎叶绿体中淀粉的合成。

3. 3.

   光增强了欧洲油菜胚胎中储存储备合成的代谢活性。

抽象的

光会影响胚胎中储存储备的积累，但其分子机制尚不完全清楚。在这里，我们监测了光对欧洲油菜储备生物合成的影响通过比较在正常光照条件下生长的长角果与那些被遮蔽或遮蔽（即完全变暗）的长角果的胚胎。与对照胚胎相比，蒙面胚胎发育得更慢、重量更轻、蛋白质和脂质更少。与对照胚胎相比，它们的油体也更少且更小，并且缺乏通常合成淀粉粒的叶绿体。大多数氨基酸、碳水化合物和脂肪酸的水平在掩蔽胚中高于对照或遮蔽胚，而在掩蔽胚乳中这些代谢物的水平低于对照和遮蔽胚乳。转录组分析表明，参与光合作用（42 个基因）、氨基酸生物合成（51 个基因）、脂质代谢（61 个基因）和糖转运（13 个基因）的基因在蒙面胚胎中受到显着抑制。