Background

Glutathione (GSH) is important for plants to resist abiotic stress, and a large amount of energy is required in the process. However, it is not clear how the energy status affects the accumulation of GSH in plants under cold stress.

Results

Two rice pure lines, Zhongzao39 (ZZ39) and its recombinant inbred line 82 (RIL82) were subjected to cold stress for 48 h. Under cold stress, RIL82 suffered more damages than ZZ39 plants, in which higher increases in APX activity and GSH content were showed in the latter than the former compared with their respective controls. This indicated that GSH was mainly responsible for the different cold tolerance between these two rice plants. Interestingly, under cold stress, greater increases in contents of carbohydrate, NAD(H), NADP(H) and ATP as well as the expression levels of GSH1 and GSH2 were showed in RIL82 than ZZ39 plants. In contrast, ATPase content in RIL82 plants was adversely inhibited by cold stress while it increased significantly in ZZ39 plants. This indicated that cold stress reduced the accumulation of GSH in RIL82 plants mainly due to the inhibition on ATP hydrolysis rather than energy deficit.

Conclusion

We inferred that the energy status determined by ATP hydrolysis involved in regulating the cold tolerance of plants by controlling GSH synthesis.

中文翻译：

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ATP 水解通过调节水稻幼苗谷胱甘肽合成的可用能量来确定耐冷性

 背景

谷胱甘肽（GSH）对于植物抵抗非生物胁迫非常重要，这个过程需要大量的能量。然而，目前尚不清楚冷胁迫下能量状态如何影响植物中GSH的积累。

 结果

将两个水稻纯系中早39号（ZZ39）及其重组自交系82号（RIL82）进行冷胁迫48小时。在冷胁迫下，RIL82比ZZ39植株遭受更大的损害，其中与对照相比，ZZ39植株的APX活性和GSH含量增加幅度更大。这表明GSH是造成这两种水稻耐寒性不同的主要原因。有趣的是，在冷胁迫下，RIL82 中碳水化合物、NAD(H)、NADP(H) 和 ATP 的含量以及GSH1和GSH2的表达水平比 ZZ39 植物有更大的增加。相比之下，RIL82植物中的ATP酶含量受到冷胁迫的不利抑制，而ZZ39植物中的ATP酶含量显着增加。这表明冷胁迫减少了RIL82植物中GSH的积累，主要是由于抑制了ATP水解而不是能量不足。

 结论

我们推测ATP水解决定的能量状态通过控制GSH合成来调节植物的耐寒性。