Visualization of Ligand-Bound Ectodomain Assembly in the Full-Length Human IGF-1 Receptor by Cryo-EM Single-Particle Analysis.,Structure

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Visualization of Ligand-Bound Ectodomain Assembly in the Full-Length Human IGF-1 Receptor by Cryo-EM Single-Particle Analysis.
Structure ( IF 4.4 ) Pub Date : 2020-04-09 , DOI: 10.1016/j.str.2020.03.007
Xi Zhang ₁ , Daqi Yu ₂ , Jingchuan Sun ₃ , Yujie Wu ₃ , Junyuan Gong ₄ , Xuemei Li ₅ , Li Liu ₄ , Shan Liu ₄ , Jianbo Liu ₃ , Yulan Wu ₄ , Dongyang Li ₄ , Yinping Ma ₆ , Xu Han ₂ , Yanan Zhu ₂ , Zhaolong Wu ₂ , Yihua Wang ₂ , Qi Ouyang ₇ , Tao Wang ₈

Affiliation

School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China; Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China.
State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing 100871, China.
Institute of Systems Biology, Shenzhen Bay Laboratory, Peking University Shenzhen Graduate School, Shenzhen 518055, China.
Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China.
Electron Microscopy Laboratory, School of Physics, Peking University, Beijing 100871, China.
Computing Center of Peking University, Peking University, Beijing 100871, China.
State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, School of Physics, Peking University, Beijing 100871, China; Center for Quantitative Biology, Peking University, Beijing 100871, China.
Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China; Institute of Systems Biology, Shenzhen Bay Laboratory, Peking University Shenzhen Graduate School, Shenzhen 518055, China; Joint Laboratory for Infectious Disease Prevention and Control, Hygienic Section of Longhua Center for Disease Control and Prevention, Shenzhen 518109, China.

Tyrosine kinase receptor of insulin-like growth factor 1 receptor (IGF-1R) and insulin receptor (IR) bind to hormones, such as insulin, IGF-1, and IGF-2, and transduces the signals across the cell membrane. However, the complete structure of the receptor and the signal transduction mechanism remains unclear. Here, we report the cryo-EM structure of the ligand-bound ectodomain in the full-length human IGF-1R. We reconstructed the IGF-1R/insulin complex at 4.7 Å and the IGF-1R/IGF-1 complex at 7.7 Å. Our structures reveal that only one insulin or one IGF-1 molecule binds to and activates the full-length human IGF-1R receptor.

中文翻译：

通过低温-EM单颗粒分析可视化全长人IGF-1受体中配体结合的电子域结构。

胰岛素样生长因子1受体（IGF-1R）和胰岛素受体（IR）的酪氨酸激酶受体与诸如胰岛素，IGF-1和IGF-2的激素结合，并在细胞膜上传递信号。但是，受体的完整结构和信号转导机制仍不清楚。在这里，我们报道了全长人IGF-1R中配体结合的胞外域的cryo-EM结构。我们在4.7Å处重建了IGF-1R /胰岛素复合物，在7.7Å处重建了IGF-1R / IGF-1复合物。我们的结构揭示，只有一种胰岛素或一种IGF-1分子结合并激活全长人IGF-1R受体。

更新日期：2020-04-09

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