Radix Tetrastigma (RT) is a medicinal plant and functional food in China, distributed in various places in the south of China. Radix Tetrastigma extract (RTE) from different origins were collected and analyzed for their anti-inflammatory effects. Different RTEs showed different abilities to suppress shape deformation, decrease the nitric oxide (NO) production, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in RAW 264.7 cells. Subsequently, their bioactive components were compared, and results showed RTEs are rich in flavonoid (85.25-436.70 mg RE/g DW), polysaccharides (100.45-349.26 mg glucose/g DW), phenolic (12.92-225.40 mg GAE/g DW) and protein contents (4.429-7.719 mg/g DW). Principal component analysis (PCA) and correlation studies indicated that anti-inflammatory capacity could be more associated with total flavonoid contents. High-performance liquid chromatography (HPLC) and Ultraperformance liquid chromatography-time-of flight mass spectrometry (UPLC-TOF/MS) analysis were conducted, and results showed that rutin, isoquercitrin, kaempferol-3-O-rutinoside and astragalin were main flavonoid compounds, among them astragalin exhibited a prior protective effect, suggesting it might be responsible for RTE's excellent anti-inflammatory capacity.

中文翻译：

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来自不同来源的四柱菊提取物保护 RAW264.7 巨噬细胞免受 LPS 诱导的炎症

黄芪（RT）是我国的药用植物和功能性食品，分布于我国南方各地。收集了来自不同来源的四柱菊提取物 (RTE) 并分析了它们的抗炎作用。不同的 RTE 在 RAW 264.7 细胞中显示出不同的抑制形状变形、减少一氧化氮 (NO) 产生、诱导型一氧化氮合酶 (iNOS) 和环氧合酶-2 (COX-2) 表达的能力。随后，对它们的生物活性成分进行了比较，结果表明 RTE 富含类黄酮（85.25-436.70 mg RE/g DW）、多糖（100.45-349.26 mg 葡萄糖/g DW）、酚类（12.92-225.40 mg GAE/g DW）和蛋白质含量 (4.429-7.719 mg/g DW)。主成分分析（PCA）和相关性研究表明抗炎能力可能与总黄酮含量更相关。进行了高效液相色谱（HPLC）和超高效液相色谱-飞行时间质谱（UPLC-TOF/MS）分析，结果表明，芦丁、异槲皮苷、山奈酚-3-O-芸香苷和黄芪甲苷是主要的黄酮类化合物化合物，其中黄芪甲苷表现出先前的保护作用，表明它可能是 RTE 出色抗炎能力的原因。