Engineering of Klebsiella oxytoca for production of 2,3‐butanediol using mixed sugars derived from lignocellulosic hydrolysates,Global Change Biology Bioenergy

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Engineering of Klebsiella oxytoca for production of 2,3‐butanediol using mixed sugars derived from lignocellulosic hydrolysates
Global Change Biology Bioenergy ( IF 5.9 ) Pub Date : 2020-02-20 , DOI: 10.1111/gcbb.12674
Ji Won Cha ₁ , Seung Hoon Jang ₁ , Yong Jae Kim ₁ , Yong Keun Chang ₁ , Ki Jun Jeong _{1,

2}

Affiliation

2,3‐Butanediol (2,3‐BDO) is a promising bulk chemical owing to its high potential in industrial applications. Here, we engineered Klebsiella oxytoca for the economic production of 2,3‐BDO using mixed sugars from renewable biomass. First, to improve xylose consumption, the xylose transporter gene (xylE) was integrated into the methylglyoxal synthase A (mgsA)‐coding gene loci, and the engineered CHA004 strain showed much faster consumption of xylose than wild‐type (WT) strain with 1.4‐fold increase of overall sugar consumption rate. To further improve sugar utilization, we performed adaptive laboratory evolution for 90 days. The evolved strain (CHA006) was evaluated by cultivating it in the media containing single‐ or mixed‐sugars, and it was clearly observed that CHA006 has improved sugar consumption and 2,3‐BDO production than those of the parental strain. Finally, we demonstrated the superiority of CHA006 by culturing in two lignocellulosic hydrolysates derived from sunflower or pine tree. Particularly, in the pine tree hydrolysate containing xylose, glucose, galactose, and mannose, the CHA006 strain showed much improved consumption rates for all sugars, and 2,3‐BDO productivity (0.73 g L⁻¹ hr⁻¹) increased by 3.2‐fold compared to WT strain. We believe that the engineered CHA006 strain can be a potential host in the development of economic bioprocess for 2,3‐BDO through efficient utilization of mixed sugars derived from lignocellulosic biomass.

中文翻译：

使用木素纤维素水解产物衍生的混合糖生产克雷伯菌的技术以生产2,3-丁二醇

2,3-丁二醇（2,3-BDO）由于其在工业应用中的巨大潜力，是一种很有前途的散装化学品。在这里，我们设计了产酸克雷伯菌，使用可再生生物质中的混合糖进行经济生产2,3-BDO。首先，为了提高木糖消耗，将木糖转运蛋白基因（xylE）整合到甲基乙二醛合酶A（mgsA）编码的基因位点和经过改造的CHA004菌株显示出的木糖消耗量比野生型（WT）快得多，总糖消耗率提高了1.4倍。为了进一步提高糖的利用率，我们进行了90天的适应性实验室进化。通过在含有单糖或混合糖的培养基中进行培养来评估进化菌株（CHA006），并且可以清楚地观察到，CHA006比亲代菌株具有更高的糖耗和2,3-BDO产量。最后，我们通过在两种源自向日葵或松树的木质纤维素水解物中进行培养来证明CHA006的优越性。特别是，在含有木糖，葡萄糖，半乳糖和甘露糖的松树水解物中，CHA006菌株显示出所有糖的消耗率大大提高，并且2,3–BDO生产率（0.73 g L^-1 小时^-1）比WT菌株增加了3.2倍。我们相信，通过有效利用木质纤维素生物质衍生的混合糖，CHA006工程菌可以成为经济发展2,3-BDO生物工艺的潜在宿主。

更新日期：2020-02-20

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