To realize the in situ study of polycrystals, X‐ray diffraction (XRD) with the sample stage of slurry flow cell is used to study the crystal state of the lactose in solution directly. The influence of absolute ethyl alcohol and pH on the crystallization of lactose is investigated by the slurry flow cell. As the lactose is dispersed into absolute ethyl alcohol, the equilibrium of crystal is reconstructed, which induces crystal dissolution, nucleation formation, and crystal growth, then results in the difference of lactose diffractogram between the absolute ethyl alcohol solution and powder state. Peaks become sharper and higher in absolute ethyl alcohol than those of absolute ethyl alcohol with pH 3.5, which suggests that the crystal growth is easier in ethyl alcohol solution. The investigation of absolute ethyl alcohol and pH on the lactose crystals proves the capability of the XRD technique for in situ crystallization study.

中文翻译：

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通过浆液流动池X射线衍射原位分析溶液中的乳糖晶体

为了实现多晶的原位研究，使用浆液流动池样品台的X射线衍射（XRD）直接研究溶液中乳糖的晶体状态。通过浆液流通池研究了无水乙醇和pH值对乳糖结晶的影响。当乳糖分散在无水乙醇中时，晶体的平衡得以重建，从而导致晶体溶解，成核形成和晶体生长，然后导致无水乙醇溶液和粉末状态之间的乳糖衍射图有所不同。与pH 3.5的无水乙醇相比，无水乙醇中的峰变得更尖锐和更高，这表明在乙醇溶液中晶体的生长更容易。