Quantitative Formation of Monomeric G-Quadruplex DNA from Multimeric Structures of c-Myc Promoter Sequence.,ChemBioChem

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Quantitative Formation of Monomeric G-Quadruplex DNA from Multimeric Structures of c-Myc Promoter Sequence.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-04-08 , DOI: 10.1002/cbic.202000159
Valerie Rauser ₁ , Elmar Weinhold ₁

Affiliation

A note of caution: G‐Quadruplex (G4)‐forming DNA sequences have a tendency to form stable multimeric structures that are difficult to denature. This can be problematic for studies with synthetic oligodeoxynucleotides. We describe a method that quantitatively converts multimeric intermolecular structures of Pu27 sequence from c‐myc promoter into desired monomeric G4 by alkaline treatment and refolding.

中文翻译：

从c-Myc启动子序列的多聚结构定量形成单体G-四链体DNA。

注意事项：形成G-四链体（G4）的DNA序列倾向于形成难以变性的稳定多聚体结构。对于合成寡聚脱氧核苷酸的研究这可能是有问题的。我们描述了一种通过碱性处理和重折叠将Pu27序列的多聚体分子间结构从c-myc启动子定量转化为所需单体G4的方法。

更新日期：2020-04-08

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