Plant microbiota colonize all organs of a plant and play crucial roles including supplying nutrients to plants, stimulating seed germination, promoting plant growth, and defending plants against biotic and abiotic stress. Because of the economic importance, interactions between citrus and microbes have been studied relatively extensively, especially citrus-pathogen interactions. However, the spatial distribution of microbial taxa in citrus trees remains under-studied. In this study, Citrus reticulata cv. Chachiensis was examined for the spatial distribution of microbes by sequencing 16S rRNA genes. More than 2.5 million sequences were obtained from 60 samples collected from soil, roots, leaves, and phloem. The dominant microbial phyla from all samples were Proteobacteria, Actinobacteria and Acidobacteria. The composition and structure of microbial communities in different samples were analyzed by PCoA, CAP, Anosim and MRPP methods. Variation in microbial species between samples were analyzed and the indicator microbes of each sample group were identified. Our results suggested that the microbial communities from different tissues varied significantly and the microenvironments of tree tissues could affect the composition of its microbial community.

植物微生物群定植在植物的所有器官中，并起着至关重要的作用，包括为植物提供营养，刺激种子发芽，促进植物生长以及保护植物免受生物和非生物胁迫。由于经济上的重要性，对柑橘与微生物之间的相互作用进行了比较广泛的研究，尤其是柑橘与病原体的相互作用。然而，柑橘类群中微生物分类群的空间分布仍未得到充分研究。在这项研究中，柑橘网简历。通过对16S rRNA基因进行测序，检查了Chachiensis的微生物空间分布。从土壤，根，叶和韧皮部收集的60个样本中获得了超过250万个序列。来自所有样品的主要微生物菌群是Proteobacteria，Actinobacteria和Acidobacteria。通过PCoA，CAP，Anosim和MRPP方法分析了不同样品中微生物群落的组成和结构。分析样品之间的微生物种类变化，并鉴定每个样品组的指示微生物。我们的研究结果表明，来自不同组织的微生物群落差异很大，树木组织的微环境可能会影响其微生物群落的组成。