Lactoferrin is one of the most important components in milk. Lactoferrin is an 80 kDa iron-binding glycoprotein that involves a diverse range of biological activities. However, the antimicrobial properties are its most widely studied function. The aim of this study was to assay the antimicrobial and antibiofilm activities of bovine lactoferrin against five of the most important foodborne pathogenic bacterial strains including Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Listeria innocua, in planktonic and biofilm forms. Bovine lactoferrin was purchased, MIC and MBC were determined for the mentioned bacterial strains and the effect of lactoferrin concentrations on biofilm formation was evaluated using microtiter plate method. Based on the results, lactoferrin had a greater effect on gram-positive bacteria, L. monocytogenes and L. innocua were the most sensitive strains. MIC for L. monocytogenes, L. innocua, S. aureus and P. aeruginosa was 6, 6, 8 and 14 mg/mL, respectively, Nevertheless, MIC for gram negative E. coli was not observed, even at 16 mg/mL. The result demonstrated that MBC for L. monocytogenes, L. innocua and S. aureus were 10, 12 and 16 mg/mL, respectively. However, MBC for P. aeruginosa and E. coli were not observed for the lactoferrin concentrations evaluated. The highest biofilm formation inhibitory effects of bovine lactoferrin were against L. monocytogenes and L. innocua. To our knowledge, this is the first report on the antibiofilm activity of bovine lactoferrin against foodborne pathogenic bacteria. Our findings suggest lactoferrin to possibly use as an anti-biofilm agent in both food and pharmaceutical industries.

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评价牛乳铁蛋白对浮游生物膜和生物膜形式的食源性病原微生物的抗微生物特性（体外）

乳铁蛋白是牛奶中最重要的成分之一。乳铁蛋白是一种80 kDa的铁结合糖蛋白，涉及多种生物活性。然而，抗菌性能是其最广泛研究的功能。这项研究的目的是测定牛乳铁蛋白对五种最重要的食源性致病细菌菌株（包括铜绿假单胞菌，大肠杆菌，金黄色葡萄球菌，单核细胞增生李斯特菌和无毒李斯特菌）的抗菌和抗生物膜活性。，以浮游生物膜形式存在。购买牛乳铁蛋白，测定上述细菌菌株的MIC和MBC，并使用微量滴定板法评估乳铁蛋白浓度对生物膜形成的影响。根据结果​​，乳铁蛋白对革兰氏阳性菌有更大的影响，单核细胞增生李斯特菌和无毒李斯特菌是最敏感的菌株。单核细胞增生李斯特菌，无毒李斯特菌，金黄色葡萄球菌和铜绿假单胞菌的MIC分别为6、6、8和14 mg / mL，但是即使在16 mg / mL的情况下，也未观察到革兰氏阴性大肠杆菌的MIC。。结果表明，MBC的单增李斯特菌，无害利斯特氏菌金黄色葡萄球菌和金黄色葡萄球菌分别为10、12和16 mg / mL。但是，未评估铜绿假单胞菌和大肠杆菌的MBC的乳铁蛋白浓度。牛乳铁蛋白对B的生物膜形成的抑制作用最高。单核细胞和大号。无毒的。据我们所知，这是关于牛乳铁蛋白对食源性致病菌的抗生物膜活性的首次报道。我们的发现表明，乳铁蛋白可能在食品和制药行业中用作抗生物膜剂。