Menthacarin attenuates experimental colitis.,Phytomedicine

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Menthacarin attenuates experimental colitis.
Phytomedicine ( IF 6.7 ) Pub Date : 2020-04-08 , DOI: 10.1016/j.phymed.2020.153212
Korina Alliger ₁ , Mohammad Khalil ₂ , Beatrix König ₃ , Sabrina Weisenburger ₃ , Egon Koch ₃ , Matthias Engel ₁

Affiliation

Background

Peppermint oil and caraway oil are established remedies in gastroenterological medicine because of their spasmolytic/analgesic effects.

Purpose

We investigated whether Menthacarin, a combination of both oils, exerted anti-inflammatory effects in a dextran sodium sulphate (DSS, 2%) murine model of colitis.

Study Design and Methods

C57BL/6 mice were orally administered Menthacarin in doses of 10, 30, 60, and 120 µg/g body weight (BW), and control mice received 0.2% agar, 10 µl/g BW, during 8 days of DSS-induced colitis. Colitis was monitored by BW measurements and colonoscopies. Colons of euthanised mice were excised for histological staining and ELISA measurements of the cytokines TNFα, IL-6, IL-10, IL-1β, and TGF-β.

Results

Menthacarin-treated mice compared to controls showed improved macroscopical and microscopical parameters and lower BW loss during the course of colitis. Menthacarin changed the colonic cytokine profile towards a regulatory/anti-inflammatory phenotype.

Conclusion

Menthacarin attenuates experimental colitis and may be a promising add-on therapy for the treatment of IBD.

中文翻译：

薄荷脑可减轻实验性结肠炎。

背景

薄荷油和香菜油由于具有解痉/止痛作用，因此在胃肠病医学中已被确立为治疗方法。

目的

我们研究了薄荷油（两种油的组合）是否在结肠炎的葡聚糖硫酸钠（DSS，2％）小鼠模型中发挥抗炎作用。

研究设计和方法

在DSS诱发的结肠炎8天中，以10、30、60和120μg/ g体重（BW）的剂量口服C57BL / 6小鼠薄荷脑，对照组小鼠接受0.2％琼脂，10μl/ g BW的剂量。通过BW测量和结肠镜检查监测结肠炎。切除安乐死小鼠的结肠，以进行细胞因子TNFα，IL-6，IL-10，IL-1β和TGF-β的组织学染色和ELISA测量。