The habenula complex is appreciated as a critical regulator of motivated and pathological behavioral states via its output to midbrain nuclei. Despite this, transcriptional definition of cell populations that comprise both the medial habenular (MHb) and lateral habenular (LHb) subregions in mammals remain undefined. To resolve this, we performed single-cell transcriptional profiling and highly multiplexed in situ hybridization experiments of the mouse habenula complex in naive mice and those exposed to an acute aversive stimulus. Transcriptionally distinct neuronal cell types identified within the MHb and LHb, were spatially defined, differentially engaged by aversive stimuli, and had distinct electrophysiological properties. Cell types identified in mice also displayed a high degree of transcriptional similarity to those previously described in zebrafish, highlighting the well-conserved nature of habenular cell types across the phylum. These data identify key molecular targets within habenular cell types and provide a critical resource for future studies.

ben复合体通过其输出到中脑核而被认为是动机和病理行为状态的关键调节剂。尽管如此，在哺乳动物中既包含内侧唇形（MHb）又包含外侧唇形（LHb）子区域的细胞群体的转录定义仍然不确定。为了解决这个问题，我们进行了单细胞转录分析并高度原位复用幼稚小鼠和暴露于急性厌恶刺激的小鼠哈贝努尔复合体的杂交实验。在MHb和LHb中鉴定出的转录上不同的神经元细胞类型，在空间上被定义，通过厌恶性刺激而不同地参与，并具有不同的电生理特性。在小鼠中鉴定出的细胞类型也与先前在斑马鱼中描述的那些细胞显示出高度的转录相似性，突显了贯穿整个门的ha形细胞类型的保守性。这些数据确定了唇形细胞类型内的关键分子靶标，并为将来的研究提供了重要资源。