Study of the Human Albumin Role in the Formation of a Bacterial Biofilm on Urinary Devices Using QCM-D,ACS Applied Bio Materials

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Study of the Human Albumin Role in the Formation of a Bacterial Biofilm on Urinary Devices Using QCM-D
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-04-08 , DOI: 10.1021/acsabm.0c00286
Cristina García-Bonillo , Robert Texidó ₁ , Guillermo Reyes-Carmenaty , Joan Gilabert-Porres ₁ , Salvador Borrós ₁

Affiliation

Catheter-associated urinary tract infections (CAUTIs) are the most common health care-associated infections due to rapid bacterial colonization+ and biofilm formation in urinary catheters. This behavior has been extensively documented in medical devices. However, there is a few literature works on CAUTI providing a model that allows the exhaustive study of biofilm formation in a urinary environment. The development of an effective model would be helpful to identify the factors that promote the biofilm formation and identify strategies to avoid it. In this work, we have developed a model to test biofilm formation on urinary medical device surfaces by simulating environmental and physical conditions using a quartz crystal microbalance with dissipation (QCM-D) module with an uropathogenic strain. Moreover, we used the developed model to study the role of human albumin present in artificial urine at high concentrations because of renal failure or heart-diseases in patients. Despite model limitations using artificial urine, these tests show that human albumin can be considered as a promoter of biofilm formation on hydrophobic surfaces, being a possible risk factor to developing a CAUTI.

中文翻译：

使用 QCM-D 研究人白蛋白在泌尿装置细菌生物膜形成中的作用

导管相关性尿路感染 (CAUTI) 是最常见的与医疗保健相关的感染，这是由于导尿管中的细菌快速定植+和生物膜形成。这种行为已在医疗设备中得到广泛记录。然而，有一些关于 CAUTI 的文献作品提供了一个模型，可以对泌尿环境中的生物膜形成进行详尽的研究。有效模型的开发将有助于确定促进生物膜形成的因素并确定避免它的策略。在这项工作中，我们开发了一个模型，通过使用具有耗散功能的石英晶体微天平 (QCM-D) 模块与泌尿致病菌株模拟环境和物理条件，来测试泌尿医疗器械表面的生物膜形成。而且，我们使用开发的模型来研究由于肾功能衰竭或心脏病患者的高浓度人工尿液中存在的人白蛋白的作用。尽管使用人工尿液的模型存在局限性，但这些测试表明，人白蛋白可被视为疏水表面上生物膜形成的促进剂，是发展 CAUTI 的可能风险因素。

更新日期：2020-04-08

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