Genome‐wide nuclear data confirm two species in the Alpine endemic land snail Noricella oreinos s.l. (Gastropoda, Hygromiidae),Journal of Zoological Systematics and Evolutionary Research

当前位置： X-MOL 学术 › J. Zool. Syst. Evol. Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Genome‐wide nuclear data confirm two species in the Alpine endemic land snail Noricella oreinos s.l. (Gastropoda, Hygromiidae)
Journal of Zoological Systematics and Evolutionary Research ( IF 2.0 ) Pub Date : 2020-01-24 , DOI: 10.1111/jzs.12362
Sonja Bamberger _{1,

2} , Michael Duda ₃ , Andreas Tribsch ₄ , Elisabeth Haring _{1,

5} , Helmut Sattmann ₃ , Oliver Macek ₁ , Matthias Affenzeller ₄ , Luise Kruckenhauser ₁

Affiliation

The Austrian endemic land snail species Noricella oreinos (formerly Trochulus oreinos) occurs in the Northeastern Calcareous Alps at high elevations. Two morphologically highly similar subspecies N. o. oreinos and N. o. scheerpeltzi have been described. First analyses of mitochondrial and nuclear marker sequences indicated a high genetic divergence between them. In the present study, we aimed to assess gene flow between the two subspecies which should help to re‐evaluate their taxonomic status. Sequence data and amplified fragment length polymorphism (AFLP) markers of 255 Noricella specimens covering the whole distribution range were analyzed. A clear geographic separation was found within the potential contact zone, the Haller Mauern mountain range. Samples of all western sites were part of the clade representing N. o. scheerpeltzi and almost all samples from the eastern sites clustered with N. o. oreinos. However, within two sampling sites of the eastern Haller Mauern, a few individuals possessed a COI sequence matching the N. o. oreinos clade whereas at the ITS2 locus they were heterozygous possessing the alleles of both taxa. Contrary to the ITS2 results indicating historical and/or ongoing hybridization, AFLP analyses of 202 individuals confirmed a clear separation of the two taxa congruent with the mitochondrial data. Although they occur on the same mountain range without any physical barrier, no indication of ongoing gene flow between the two taxa was found. Thus, we conclude that the two taxa are separate species N. oreinos and N. scheerpeltzi.

中文翻译：

全基因组核数据证实了阿尔卑斯特有的陆地蜗牛Noricella oreinos sl（腹足纲，Hygromiidae）中的两个物种

奥地利特有的陆地蜗牛物种Noricella oreinos（以前称为Trochulus oreinos）出现在东北部钙质阿尔卑斯山的高海拔地区。两个形态高度相似的亚种N. o. oreinos和N.o. scheerpeltzi已被描述。对线粒体和核标记序列的首次分析表明它们之间存在高度遗传差异。在本研究中，我们的目的是评估两个亚种之间的基因流，这将有助于重新评估它们的分类地位。对涵盖整个分布范围的255 个诺里氏菌样本的序列数据和扩增片段长度多态性 (AFLP) 标记进行了分析。在潜在接触区 Haller Mauern 山脉内发现了明显的地理分离。所有西部遗址的样本都是代表 N. o. 的分支的一部分。scheerpeltzi和几乎所有来自东部地点的样本都与N. o. 聚集在一起。奥利诺斯。然而，在 Haller Mauern 东部的两个采样点内，少数个体拥有与 N. o.匹配的COI序列。oreinos clade，而在ITS2基因座上，它们是杂合的，拥有两个类群的等位基因。与表明历史和/或正在进行的杂交的ITS2结果相反，对 202 名个体的 AFLP 分析证实了与线粒体数据一致的两个类群的明显分离。尽管它们出现在同一山脉上，没有任何物理障碍，但没有发现两个类群之间持续基因流动的迹象。因此，我们得出结论，这两个类群是不同的物种N. oreinos和N. scheerpeltzi。

更新日期：2020-01-24

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11