Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide and the third most frequent cause of cancer‐related death. The IRX5 transcription factor plays a different role in multiple cancers and contributes to the development of many tumours. However, little is known about the molecular mechanisms of IRX5 in HCC. In this study, we found that IRX5 was abnormally upregulated in HCC tissues compared with adjacent normal tissues. IRX5 promoted HCC cell proliferation and upregulated the expression of cyclin D1 and knockdown of IRX5 suppressed tumorigenicity in vivo . Furthermore, knockdown of IRX5 increased p53 and Bax expression and decreased Bcl‐2 expression. Thus, IRX5 suppressed apoptosis in HCC cells by inhibiting the p53 signalling pathway, indicating its role as a treatment target for HCC.

中文翻译：

---

转录因子IRX5通过调节p53信号通路促进肝细胞癌的增殖并抑制细胞凋亡。

肝细胞癌（HCC）是全球第五大最常见的癌症，也是癌症相关死亡的第三大最常见原因。IRX5转录因子在多种癌症中起着不同的作用，并且有助于许多肿瘤的发展。但是，关于肝癌中IRX5的分子机制知之甚少。在这项研究中，我们发现与邻近的正常组织相比，IRC5在HCC组织中异常上调。IRX5促进肝癌细胞增殖，上调cyclin D1的表达，抑制IRX5抑制体内致瘤性。此外，IRX5的敲低增加了p53和Bax表达，并降低了Bcl-2表达。因此，IRX5通过抑制p53信号通路来抑制HCC细胞凋亡，表明其作为HCC治疗靶标的作用。