This study was aimed to explore the expression and biological function of circRNA_0005075 in gastric cancer (GC) progression and its underlying mechanism. First, the expression level of circRNA_0005075 and microRNA‐431 (miR‐431) in GC tissues were detected with the quantitative real‐time polymerase chain reaction. In addition, after down‐regulated the circRNA_0005075 expression by plasmid transfection in GC cells, the Cell Counting Kit‐8 (CCK‐8), EDU, transwell assay were conducted to evaluate the function of circRNA_0005075 or miR‐431 on cell proliferation, metastasis in vitro. Moreover, p53 and Epithelial‐mesenchymal transition (EMT) pathway related proteins were also measured with western blotting. Then, our data revealed that CircRNA_0005075 was found to be significantly up‐regulated in GC tissues as well as GC cell lines, and the GC patients with higher CircRNA_0005075 expression were more likely to have poor outcomes. Down‐regulation of CircRNA_0005075 could significantly suppress the GC cell proliferation and cell metastasis ability, while the addition of miR‐431 inhibitors could counteract this effect. Importantly, we discovered that the silencing of circRNA_0005075 could weaken the micro‐RNA sponge function for miR‐431, and then upregulate the expression of p53 and forbid the EMT signalling pathway, and finally suppress the tumourigenesis of GC. To sum up, CircRNA_0005075 could inhibit cell growth and metastasis of GC through regulating the miR‐431/p53/EMT axis.

中文翻译：

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CircRNA_0005075通过调节miR-431 / p53 /上皮间质转化轴抑制胃癌的发生

本研究旨在探讨circRNA_0005075在胃癌（GC）进展中的表达及其生物学功能及其潜在机制。首先，通过实时定量聚合酶链反应检测GC组织中circRNA_0005075和microRNA-431（miR-431）的表达水平。此外，通过质粒转染在GC细胞中下调了circRNA_0005075的表达后，进行了Cell Counting Kit-8（CCK-8），EDU和transwell分析以评估circRNA_0005075或miR-431在细胞增殖，转移中的作用。体外。此外，p53和上皮-间质转化（EMT）途径相关的蛋白质也用Western印迹法检测。然后，我们的数据表明，发现CircRNA_0005075在GC组织和GC细胞系中明显上调，CircRNA_0005075表达较高的GC患者更有可能出现不良预后。下调CircRNA_0005075可以显着抑制GC细胞增殖和细胞转移能力，而添加miR-431抑制剂则可以抵消这种作用。重要的是，我们发现沉默circRNA_0005075可能会削弱miR-431的微RNA海绵功能，然后上调p53的表达并禁止EMT信号通路，并最终抑制GC的肿瘤发生。综上所述，CircRNA_0005075可以通过调节miR-431 / p53 / EMT轴来抑制细胞生长和转移。而添加miR-431抑制剂可以抵消这种作用。重要的是，我们发现沉默circRNA_0005075可能会削弱miR-431的微RNA海绵功能，然后上调p53的表达并禁止EMT信号通路，并最终抑制GC的肿瘤发生。综上所述，CircRNA_0005075可以通过调节miR-431 / p53 / EMT轴来抑制细胞生长和转移。而添加miR-431抑制剂可以抵消这种作用。重要的是，我们发现沉默circRNA_0005075可能会削弱miR-431的微RNA海绵功能，然后上调p53的表达并禁止EMT信号通路，并最终抑制GC的肿瘤发生。综上所述，CircRNA_0005075可以通过调节miR-431 / p53 / EMT轴来抑制细胞生长和转移。