Dihydromethysticin (DHM), a natural compound derived from Kava, has been reported to be effective against mental disorders and some malignant tumors. However, little is known about the inhibitory effect of DHM on colorectal cancer (CRC). First, we examined the impact of DHM on human colon cancer cell lines, which demonstrated that DHM inhibits proliferation, migration, and invasion and promotes apoptosis and cell cycle arrest in colon cancer cells in vitro. Using small hairpin RNA, we inhibited nucleotide-oligomerization domain-like receptor subfamily C3 (NLRC3)/phosphoinositide 3-kinase (PI3K) pathway to elucidate the partial signaling of DHM-mediated tumor suppression. Additionally, using an ectopic human CRC model, we verified whether DHM inhibits tumor growth and angiogenesis via the NLRC3/PI3K pathway in vivo. Overall, DHM showed an inhibitory effect on CRC by altering cell proliferation, migration, invasion, apoptosis, cell cycle, and angiogenesis, possibly via the NLRC3/PI3K pathway. Thus, DHM may be a promising candidate for CRC therapy.

中文翻译：

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来自Kava的天然分子Dihydromethysticin通过NLRC3 / PI3K途径抑制结肠直肠癌的生长。

据报道，二氢甲基葡萄球菌素（DHM）是一种由卡瓦（Kava）衍生的天然化合物，可有效治疗精神疾病和某些恶性肿瘤。然而，关于DHM对结直肠癌（CRC）的抑制作用知之甚少。首先，我们检查了DHM对人结肠癌细胞系的影响，这表明DHM在体外抑制了结肠癌细胞的增殖，迁移和侵袭，并促进了细胞凋亡和细胞周期阻滞。使用小的发夹RNA，我们抑制了核苷酸寡聚化域样受体亚家族C3（NLRC3）/磷酸肌醇3激酶（PI3K）通路，以阐明DHM介导的肿瘤抑制的部分信号。此外，使用异位人类CRC模型，我们验证了DHM是否通过NLRC3 / PI3K途径在体内抑制肿瘤生长和血管生成。总体，DHM可能通过NLRC3 / PI3K途径改变细胞增殖，迁移，侵袭，凋亡，细胞周期和血管生成，从而对CRC产生抑制作用。因此，DHM可能是CRC疗法的有希望的候选者。