Over the years, microRNA‐20b‐3p (miR‐20b‐3p) has been found to play an essential role in human diseases; we aimed to investigate the effect of miR‐20b‐3p on the progression of diabetic retinopathy (DR). The DR rat models were established by streptozotocin injection and treated with miR‐20b‐3p mimics, silenced, or overexpressed thioredoxin‐interacting protein (TXNIP). Afterward, the expression of miR‐20b‐3p and TXNIP, visual function, inflammatory factors, microvascular injury, vascular permeability, cell apoptosis, and angiogenesis in rats' retinal tissues were assessed. The target relation between miR‐20b‐3p and TXNIP was confirmed by dual luciferase reporter gene assay. MiR‐20b‐3p was poorly expressed while TXNIP was highly expressed in DR rats' retinal tissues. Elevated miR‐20b‐3p and inhibited TXNIP promoted the visual function, and restricted the inflammatory reaction, microvascular injury, vascular permeability, cell apoptosis, and angiogenesis in DR rats, thereby decelerating the development of DR. Furthermore, TXNIP was targeted by miR‐20b‐3p. We have found in this study that elevated miR‐20b‐3p could repress the levels of inflammatory factors by inhibiting TXNIP, thus attenuating the pathology of retina in DR rats, which provided new candidates for DR treatment.

中文翻译：

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升高的 microRNA-20b-3p 和降低的硫氧还蛋白相互作用蛋白通过抑制 NLRP3 炎症小体改善糖尿病视网膜病变进展

多年来，已发现 microRNA-20b-3p (miR-20b-3p) 在人类疾病中发挥重要作用；我们旨在研究 miR-20b-3p 对糖尿病视网膜病变 (DR) 进展的影响。通过注射链脲佐菌素建立 DR 大鼠模型，并用 miR-20b-3p 模拟物、沉默或过表达的硫氧还蛋白相互作用蛋白 (TXNIP) 处理。随后，评估了大鼠视网膜组织中 miR-20b-3p 和 TXNIP 的表达、视觉功能、炎症因子、微血管损伤、血管通透性、细胞凋亡和血管生成。miR-20b-3p 和 TXNIP 之间的靶标关系通过双荧光素酶报告基因检测得到证实。MiR-20b-3p 在 DR 大鼠的视网膜组织中低表达而 TXNIP 高表达。升高的 miR-20b-3p 和抑制 TXNIP 促进了视觉功能，并限制 DR 大鼠的炎症反应、微血管损伤、血管通透性、细胞凋亡和血管生成，从而减缓 DR 的发展。此外，TXNIP 被 miR-20b-3p 靶向。我们在这项研究中发现，升高的 miR-20b-3p 可以通过抑制 TXNIP 来抑制炎症因子的水平，从而减轻 DR 大鼠视网膜的病理，这为 DR 治疗提供了新的候选者。