Baicalein has been widely studied and showed a potent activity against pancreatic cancer in both in vivo and in vitro studies. Little is known regarding the effects of Skullcapflavone I (SFI), despite they have similar structures. So, this study was to explore the function of SFI on human pancreatic cancer. Panc‐1 cells were transfected with miR‐23a precursor, miR‐23a inhibitor or the negative controls, and subsequently treated by SFI. Cell viability, Bromodeoxyuridine (BrdU)‐positive cell rate, apoptosis, migration, invasion, and related protein expression were assessed by utilizing Cell Counting Kit‐8 (CCK‐8), BrdU staining, apoptosis assessment, transwell assay, and western blot. SFI significantly reduced the proliferation, migration, and invasion, as well as induced apoptosis of Panc‐1 cells. MiR‐23a, miR‐21, and miR‐155 were lowly expressed while miR‐145 and miR‐146a were highly expressed in SFI‐treated cell. Of note, the antitumor effects of SFI were promoted by miR‐23a suppression whereas attenuated by miR‐23a overexpression. JAK/STAT and MAPK pathways were inhibited by SFI. Also, the pathway inhibition in SFI‐treated cells was reversed by miR‐23a overexpression. SFI might be a promising anti‐pancreatic cancer agent by inhibiting cancer cells growth and motility. The anticancer activities of SFI might be through downregulation of miR‐23a, as well as inhibition of JAK/STAT and MAPK pathways.

中文翻译：

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Skullcapflavone I 通过靶向 miR-23a 具有有效的抗胰腺癌活性

黄芩素已被广泛研究，并在体内和体外研究中显示出有效的抗胰腺癌活性。尽管 Skullcapflavone I (SFI) 具有相似的结构，但对它们的作用知之甚少。因此，本研究旨在探讨SFI对人胰腺癌的作用。用 miR-23a 前体、miR-23a 抑制剂或阴性对照转染 Panc-1 细胞，然后用 SFI 处理。通过使用细胞计数试剂盒-8 (CCK-8)、BrdU 染色、细胞凋亡评估、transwell 测定和蛋白质印迹评估细胞活力、溴脱氧尿苷 (BrdU) 阳性细胞率、细胞凋亡、迁移、侵袭和相关蛋白质表达。SFI显着降低了Panc-1细胞的增殖、迁移和侵袭，并诱导其凋亡。miR-23a、miR-21、在 SFI 处理的细胞中，miR-155 和 miR-155 低表达，而 miR-145 和 miR-146a 高表达。值得注意的是，SFI 的抗肿瘤作用被 miR-23a 抑制促进，而被 miR-23a 过表达减弱。SFI 抑制 JAK/STAT 和 MAPK 通路。此外，SFI 处理细胞中的通路抑制被 miR-23a 过表达逆转。通过抑制癌细胞的生长和运动，SFI 可能是一种很有前途的抗胰腺癌药物。SFI 的抗癌活性可能是通过下调 miR-23a 以及抑制 JAK/STAT 和 MAPK 通路实现的。SFI 处理的细胞中的通路抑制被 miR-23a 过表达逆转。通过抑制癌细胞的生长和运动，SFI 可能是一种很有前途的抗胰腺癌药物。SFI 的抗癌活性可能是通过下调 miR-23a 以及抑制 JAK/STAT 和 MAPK 通路实现的。SFI 处理的细胞中的通路抑制被 miR-23a 过表达逆转。通过抑制癌细胞的生长和运动，SFI 可能是一种很有前途的抗胰腺癌药物。SFI 的抗癌活性可能是通过下调 miR-23a 以及抑制 JAK/STAT 和 MAPK 通路实现的。