The purpose of this study was to describe the distinct regional distribution patterns of expression of the α7 and α4 subunits of nicotinic acetylcholine receptors (nAChRs) and their left‐right lateralisation in the rat hippocampus during the first 2 weeks of postnatal (P) development. Eighteen male pups were randomly divided into three groups: P0, P7, and P14. After removing the newborn brains, real‐time polymerase chain reaction, western blot, and immunohistochemistry techniques were used to evaluate expression of the receptors. Results indicated that the expression profile of these receptors were time‐ and spatially dependent. A significant increase was observed in the distribution of α7 and α4 nAChR subunits in the developing rat hippocampus from P0 to P7 (p < .001); however, there was a significant decrease from P7 to P14 (p < .05). As a spatial effect, the highest optical density (OD) was observed in the CA3 and CA2 regions of the hippocampus, while the lowest OD was in the dentate gyrus. Moreover, the distribution of α7 and α4 nAChR subunits in the left hippocampus was significantly higher than their counterparts in the right (p < .05). From these data, the expression patterns of α7 and α4 nAChR subunits exhibited left‐right asymmetry in the developing rat hippocampus.

中文翻译：

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发育中的大鼠海马中烟碱乙酰胆碱受体 (nAChRs) α7 和 α4 亚基的发育调节和侧化

本研究的目的是描述烟碱乙酰胆碱受体 (nAChRs) 的 α7 和 α4 亚基表达的不同区域分布模式及其在出生后 (P) 发育的前 2 周内在大鼠海马中的左右偏侧化。18 只雄性幼崽被随机分为三组：P0、P7 和 P14。取出新生儿大脑后，使用实时聚合酶链反应、蛋白质印迹和免疫组织化学技术评估受体的表达。结果表明这些受体的表达谱具有时间和空间依赖性。观察到发育中的大鼠海马中 α7 和 α4 nAChR 亚基的分布从 P0 到 P7 显着增加（p < .001）；然而，从 P7 到 P14 显着降低（p < .05）。作为空间效应，在海马的 CA3 和 CA2 区域观察到最高的光密度 (OD)，而在齿状回中最低的 OD。此外，左侧海马中 α7 和 α4 nAChR 亚基的分布显着高于右侧（p < .05）。根据这些数据，α7 和 α4 nAChR 亚基的表达模式在发育中的大鼠海马中表现出左右不对称。