Candida hispaniensis is an oleaginous yeast with a great potential for production of single cell oil according to its naturally high lipid accumulation capacity. Its unusual small genome size trait is also attractive for fundamental research on genome evolution. Our physiological study suggests a great potential for lipid production, reaching 224 mg/g of cell dry weight in glucose minimum medium. C. hispaniensis is also able to secrete up to 34.6 mg/L of riboflavin promising further riboflavin production improvements by cultivation optimization and genetic engineering. However, while its genome sequence has been released very recently, no genetic tools have been described up to now for this yeast limiting its use for fundamental research and for exploitation in an industrial biotechnology. We report here the first genetic modification of C. hispaniensis by introducing a heterologous invertase allowing the growth on sucrose using a biolistic transformation approach using a dedicated vector.

中文翻译：

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改造西班牙念珠菌（Candida hispaniensis），一种很有前途的产油和发黄酵母。

西班牙念珠菌是一种油质酵母，根据其天然的高脂质积累能力，它具有生产单细胞油的巨大潜力。其不寻常的小基因组大小特征对于基因组进化的基础研究也很有吸引力。我们的生理研究表明，脂质的生产潜力巨大，在葡萄糖最低培养基中，其干重可达224 mg / g。西班牙念珠菌还能够分泌高达34.6 mg / L的核黄素，有望通过培养优化和基因工程进一步改善核黄素的生产。然而，尽管其基因组序列已被释放，但到目前为止，尚未有针对该酵母的遗传工具被描述，从而限制了其在基础研究和工业生物技术中的应用。我们在这里报告了C. hispaniensis的第一个遗传修饰，方法是引入异源转化酶，使用专用载体通过射弹转化方法在蔗糖上生长。