当前位置: X-MOL 学术Scand. J. Immunol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
TRIM32 promotes inflammatory responses in rheumatoid arthritis fibroblast-like synoviocytes.
Scandinavian Journal of Immunology ( IF 4.1 ) Pub Date : 2020-03-20 , DOI: 10.1111/sji.12876
Tian Liang 1 , Min Song 1 , Kewu Xu 2 , Chenglong Guo 2 , Hongbin Xu 2 , Hongwei Zhang 2 , Lanping Xu 3
Affiliation  

Rheumatoid arthritis (RA) is a worldwide autoimmune disease. The study of its aetiology and mechanism has always been a focus topic in medicine. This research was designed to investigate the effect of E3 ubiquitin ligase tripartite motif protein 32 (TRIM32) in rheumatoid arthritis (RA). We found in fibroblast-like synoviocytes (FLS) of RA patients, the expression of TRIM32 was significantly increased compared with its expression in osteoarthritis (OA) patients FLS. A widely used pro-inflammatory stimuli tumour necrosis factor-alpha (TNF-α) was found to promote TRIM32 expression in a time-dependent manner. Furthermore, we observed that overexpression of TRIM32 aggravated the production of pro-inflammatory cytokines in FLS, silencing of TRIM32 showed the consistent results. In addition, TRIM32 was found to activate nuclear factor κB (NF-κB) signalling pathway, and TRIM32 could interact with TNF receptor-associated factor 2 (TRAF2) to promote the K63-linked polyubiquitination of TRAF2 in RA-FLS. In conclusion, we suggested that TRIM32 as a positive regulator of inflammatory responses in RA-FLS.

中文翻译:

TRIM32促进类风湿关节炎成纤维细胞样滑膜细胞的炎症反应。

类风湿关节炎(RA)是一种全球性自身免疫性疾病。对其病因和作用机理的研究一直是医学界的研究热点。本研究旨在研究E3泛素连接酶三重基序蛋白32(TRIM32)在类风湿关节炎(RA)中的作用。我们发现在RA患者的成纤维样滑膜细胞(FLS)中,TRIM32的表达与其在骨关节炎(OA)患者FLS中的表达相比明显增加。发现广泛使用的促炎性刺激肿瘤坏死因子-α(TNF-α)以时间依赖的方式促进TRIM32表达。此外,我们观察到,TRIM32的过表达加重了FLS中促炎细胞因子的产生,TRIM32的沉默显示了一致的结果。此外,发现TRIM32激活核因子κB(NF-κB)信号传导途径,并且TRIM32可以与TNF受体相关因子2(TRAF2)相互作用,促进RA-FLS中TRAF2的K63连接多聚泛素化。总之,我们建议TRIM32作为RA-FLS中炎症反应的正调节剂。
更新日期:2020-03-07
down
wechat
bug