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Multilocus sequence typing of Escherichia coli isolates from urinary tract infection patients and from fecal samples of healthy subjects in a college community.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-03-18 , DOI: 10.1002/mbo3.1032
Yusuke Matsui 1 , Yuan Hu 2 , Julia Rubin 1 , Reginara Souza de Assis 2 , Joy Suh 1 , Lee W Riley 1
Affiliation  

Community‐acquired urinary tract infections (UTIs) are one of the most common bacterial infections worldwide. Escherichia coli is the most common cause of community‐acquired UTI. In general, UTI results from E. coli in the intestine that enters the bladder via the urethra. However, whether these E. coli strains that cause UTI represent members of the intestinal commensal E. coli or a distinct subgroup of pathogenic E. coli remains unestablished. Here, we analyzed E. coli isolates from fecal samples of healthy volunteers and urine samples of UTI patients obtained from a university‐affiliated health center. The E. coli isolates were genotyped by multilocus sequence typing (MLST). From May to October 2018, we analyzed 89 E. coli isolates from 76 (75%) rectal swabs from 113 unique healthy volunteers. We also analyzed 106 (27%) E. coli isolates from 398 unique urine samples collected between August and October 2018. Fecal and urine E. coli isolates each contained 31 distinct sequence types (STs). Nine STs were shared by fecal and urine E. coli isolates, which accounted for approximately 50% of urine isolates typed by MLST. Among the shared genotypes, ST10 and ST131 were significantly more frequently found in fecal samples, whereas ST95 and ST127 were significantly more frequently recovered from UTI samples. ST73 was found only among urine samples. These E. coli genotypes clustered and fluctuated over time. These observations suggest that E. coli genotypes found to cause UTI transiently colonize the intestine and that their primary reservoir may reside outside of the human intestine.

中文翻译:

大肠杆菌分离株的多基因座序列分型,来自大学社区的尿路感染患者和健康受试者的粪便样本。

社区获得性尿路感染(UTI)是全球最常见的细菌感染之一。大肠杆菌是社区获得性UTI的最常见原因。通常,UTI是由肠中的大肠杆菌引起的,肠中的大肠杆菌通过尿道进入膀胱。然而,导致UTI的这些大肠杆菌菌株代表肠道共生大肠杆菌的成员还是致病性大肠杆菌的不同亚群仍是未知的。在这里,我们分析了健康志愿者粪便样本中的大肠杆菌分离物以及从大学附属健康中心获得的UTI患者尿液样本。该大肠杆菌通过多基因座序列分型(MLST)对分离株进行基因分型。从2018年5月至2018年10月,我们分析了来自113位独特健康志愿者的76张(75%)直肠拭子中的89株大肠杆菌。我们还分析了2018年8月至2018年10月收集的398份独特尿液样本中的106株(27%)大肠杆菌分离物。粪便和尿液大肠杆菌分离物每种均包含31种不同的序列类型(ST)。粪便和尿液大肠杆菌共有9个ST分离株,约占MLST分型的尿分离株的50%。在共有的基因型中,粪便样品中发现ST10和ST131的频率更高,而UTI样品中发现ST95和ST127的频率更高。仅在尿液样本中发现了ST73。这些大肠杆菌基因型随时间聚集并波动。这些观察结果表明,发现引起UTI的大肠杆菌基因型瞬时在肠道内定殖,并且它们的主要贮库可能位于人肠外部。
更新日期:2020-03-18
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