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The day-to-day stability of the ruminal and fecal microbiota in lactating dairy cows.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-03-16 , DOI: 10.1002/mbo3.990 Shuai Huang 1 , Shoukun Ji 1, 2 , Hui Yan 1, 2 , Yangyi Hao 1 , Jun Zhang 1 , Yajing Wang 1 , Zhijun Cao 1 , Shengli Li 1
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-03-16 , DOI: 10.1002/mbo3.990 Shuai Huang 1 , Shoukun Ji 1, 2 , Hui Yan 1, 2 , Yangyi Hao 1 , Jun Zhang 1 , Yajing Wang 1 , Zhijun Cao 1 , Shengli Li 1
Affiliation
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In this study, we examined differences between the microbiota of the ruminal fluid (DR) and feces (DF) from five lactating dairy cows over three consecutive days using 16S rRNA gene sequence‐based analysis. Results showed significant differences between the microbial communities of the DR and DF. In particular, the relative abundance of the phyla Firmicutes and Actinobacteria was significantly lower (q < 0.001) in DR compared with DF, while the relative abundance of Bacteroidetes was significantly higher in DF than that of DR (q < 0.001). A significantly higher relative abundance of the genera Bifidobacterium, 5‐7N15, Clostridium, Epulopiscium, SMB53, Turicibacter, Dorea, Roseburia, and Akkermansia was observed in the DF, while a higher relative abundance of the genera Prevotella, Butyrivibrio, CF231, RFN20, and Succiniclasticum was observed in the DR. A further analysis using the functional prediction program PICRUSt showed that sequences belonging to the 5‐7N15, Akkermansia, Bifidobacterium, Clostridium, Dorea, Epulopiscium, Roseburia, and Turicibacter were significantly and positively correlated with glycan biosynthesis and metabolism, while CF231, Prevotella, RFN20, and Succiniclasticum were significantly and positively correlated with amino acid, lipid, carbohydrate, other amino acid, cofactors, and vitamins metabolism. No significant differences were observed across the three consecutive days in either the DR or DF ecosystems, with no significant differences in the diversity or abundance at the phylum and genus levels suggested that there is a limited day‐to‐day variability in the gut microbiota.
中文翻译：
泌乳奶牛瘤胃和粪便微生物群的日常稳定性。
在这项研究中，我们使用基于16S rRNA基因序列的分析方法，连续三天检查了五头泌乳奶牛的瘤胃液（DR）和粪便（DF）的微生物区系之间的差异。结果显示，DR和DF的微生物群落之间存在显着差异。特别是， 与DF相比，DR中的菌门菌和放线菌的相对丰度显着较低（q <0.001），而DF中的拟杆菌的相对丰度显着高于DR（q <0.001）。双歧杆菌属（5-7N15），梭菌（Clostridium），Epulopiscium，SMB53，Turicibacter，Dorea，罗斯氏和Akkermansia在DF中观察到，而属的较高的相对丰度普雷沃，丁酸，CF231，RFN20和Succiniclasticum在DR进行了观察。使用功能预测程序的进一步分析PICRUSt表明序列属于5-7N15，Akkermansia，双歧杆菌，梭状芽孢杆菌，Dorea，Epulopiscium，罗斯氏和Turicibacter与聚糖的生物合成和代谢的显著和正相关，而CF231，普氏菌，RFN20，和Succiniclasticum被显著和正与氨基酸，脂质，碳水化合物，其他氨基酸，辅因子和维生素代谢相关。在DR或DF生态系统中，连续三天均未观察到显着差异，门和属水平的多样性或丰度也无显着差异，这表明肠道菌群的日常变异有限。
更新日期：2020-03-16
中文翻译：
泌乳奶牛瘤胃和粪便微生物群的日常稳定性。
在这项研究中，我们使用基于16S rRNA基因序列的分析方法，连续三天检查了五头泌乳奶牛的瘤胃液（DR）和粪便（DF）的微生物区系之间的差异。结果显示，DR和DF的微生物群落之间存在显着差异。特别是， 与DF相比，DR中的菌门菌和放线菌的相对丰度显着较低（q <0.001），而DF中的拟杆菌的相对丰度显着高于DR（q <0.001）。双歧杆菌属（5-7N15），梭菌（Clostridium），Epulopiscium，SMB53，Turicibacter，Dorea，罗斯氏和Akkermansia在DF中观察到，而属的较高的相对丰度普雷沃，丁酸，CF231，RFN20和Succiniclasticum在DR进行了观察。使用功能预测程序的进一步分析PICRUSt表明序列属于5-7N15，Akkermansia，双歧杆菌，梭状芽孢杆菌，Dorea，Epulopiscium，罗斯氏和Turicibacter与聚糖的生物合成和代谢的显著和正相关，而CF231，普氏菌，RFN20，和Succiniclasticum被显著和正与氨基酸，脂质，碳水化合物，其他氨基酸，辅因子和维生素代谢相关。在DR或DF生态系统中，连续三天均未观察到显着差异，门和属水平的多样性或丰度也无显着差异，这表明肠道菌群的日常变异有限。
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