AIMS The role of the immune response to cyathostomin infections in horses remains unknown. Intestinal goblet cell hyperplasia has previously been noted as a component in cyathostomin infection; however, the function is unclear. The goal of this study was to evaluate the local and systemic gene expression to cyathostomin infections following larvicidal treatment and explore their relation to goblet cells. METHODS AND RESULTS Thirty-six ponies with naturally acquired cyathostomin infections were randomly allocated into three groups: fenbendazole-treated (10 mg/kg PO 5 days), moxidectin-treated (0.4 mg/kg PO once) and untreated control. Whole blood from all horses was collected weekly, and tissue samples from the large intestine collected during necropsy at 2 and 5 weeks post-treatment (WPT). Gene expression of interleukin (IL)-4, IL-5, IL-6, IL-10, IL-13, IL-17A, IL-22, IFN-γ, resistin-like molecule beta (RELM-β), Mucin 2 (MUC2) and tumour necrosis factor (TNF)-α was measured using qRT-PCR. There were statistically significant linear correlations between luminal worm burdens and MUC2 (r = -.2358) and RELM-β (r = -.2261). CONCLUSION This suggests an active role of immune system post-treatment in parasite expulsion, specifically in goblet cells, and that the organs respond differently to treatment and the larvae themselves. This may have implications in the disease process and treatment.

中文翻译：

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马赛艇总蛋白感染和幼虫驱虫药治疗中细胞因子和杯状细胞基因的表达。

目的免疫反应对马狗中的鞘管蛋白感染的作用仍然未知。肠道杯状细胞增生以前已被认为是泛藻蛋白感染的一个组成部分。但是，功能尚不清楚。这项研究的目的是评估杀幼虫剂处理后对yaostostomin感染的局部和全身基因表达，并探讨它们与杯状细胞的关系。方法和结果将三十六只自然感染犬小脑蠕虫感染的小马随机分为三组：芬苯达唑治疗（10 mg / kg PO 5天），莫昔菌素治疗（0.4 mg / kg PO 5次）和未治疗对照。每周收集所有马的全血，在尸检期间（治疗后2周和5周）从大肠中收集组织样本。白介素（IL）-4，IL-5，IL-6，IL-10，使用qRT-PCR测量IL-13，IL-17A，IL-22，IFN-γ，抵抗素样分子β（RELM-β），粘蛋白2（MUC2）和肿瘤坏死因子（TNF）-α。管腔蠕虫负担与MUC2（r = -.2358）和RELM-β（r = -.2261）之间存在统计学上显着的线性相关性。结论这表明免疫系统后处理在驱逐寄生虫（特别是杯状细胞）中起积极作用，并且器官对处理和幼虫本身的反应也不同。这可能对疾病的过程和治疗有影响。结论这表明免疫系统后处理在驱逐寄生虫（特别是杯状细胞）中起积极作用，并且器官对处理和幼虫本身的反应也不同。这可能对疾病的过程和治疗有影响。结论这表明免疫系统后处理在驱逐寄生虫（特别是杯状细胞）中具有积极作用，并且器官对处理和幼虫本身的反应也不同。这可能对疾病的过程和治疗有影响。