Many pathogenicity factors are involved in the development of primary amoebic meningoencephalitis (PAM) caused by N fowleri. However, most of them are not exclusive for N fowleri and they have not even been described in other nonpathogenic Naegleria species. Therefore, the objective of this work was to identify differential proteins and protein pattern recognition between Naegleria fowleri and Naegleria lovaniensis using antibodies anti-N fowleri as strategy to find vaccine candidates against meningoencephalitis. Electrophoresis and Western blots conventional and 2-DE were performed for the identification of antigenic proteins, and these were analysed by the mass spectrometry technique. The results obtained in 2-DE gels and Western blot showed very notable differences in spot intensity between these two species, specifically those with relative molecular weight of 100, 75, 50 and 19 kDa. Some spots corresponding to these molecular weights were identified as actin fragment, myosin II, heat shock protein, membrane protein Mp2CL5 among others, with differences in theoretical post-translational modifications. In this work, we found differences in antigenic proteins between both species, proteins that could be used for a further development of vaccines against N fowleri infection.

中文翻译：

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福建鸡血藤与洛夫奈格鱼的差异蛋白质识别模式的鉴定。

N Fowleri引起的原发性阿米巴脑膜脑炎（PAM）的发展涉及许多致病因素。但是，它们中的大多数并不是N禽类所独有的，甚至在其他非致病性Naegleria物种中也没有描述。因此，这项工作的目的是使用抗N禽类抗体作为寻找抗脑膜脑炎候选疫苗的策略，来鉴定禽类和洛氏种之间的差异蛋白质和蛋白质模式识别。进行常规电泳和Western印迹和2-DE鉴定抗原蛋白，并通过质谱技术对其进行分析。在2-DE凝胶和Western印迹中获得的结果表明，这两种分子之间的斑点强度非常明显，特别是相对分子量为100、75、50和19 kDa的那些。与这些分子量相对应的一些斑点被鉴定为肌动蛋白片段，肌球蛋白II，热休克蛋白，膜蛋白Mp2CL5等，在翻译后理论修饰上存在差异。在这项工作中，我们发现两种物种之间的抗原蛋白存在差异，这些蛋白可用于进一步开发针对N Fowleri感染的疫苗。