Dengue virus (DENV) comprises of four serotypes (DENV‐1 to ‐4) and is medically one of the most important arboviruses (arthropod‐borne virus). DENV infection is a major human health burden and is transmitted between humans by the insect vector, Aedes aegypti . Ae. aegypti ingests DENV while feeding on infected humans, which traverses through its gut, haemolymph and salivary glands of the mosquito before being injected into a healthy human. During this process of transmission, DENV must interact with many proteins of the insect vector, which are important for its successful transmission. Our study focused on the identification and characterisation of interacting protein partners in Ae. aegypti to DENV. Since domain III (DIII) of envelope protein (E) is exposed on the virion surface and is involved in virus entry into various cells, we performed phage display library screening against domain III of the envelope protein (EDIII) of DENV‐2. A peptide sequence showing similarity to lachesin protein was found interacting with EDIII. The lachesin protein was cloned, heterologously expressed, purified and used for in vitro interaction studies. Lachesin protein interacted with EDIII and also with DENV. Further, lachesin protein was localised in neuronal cells of different organs of Ae. aegypti by confocal microscopy. Blocking of lachesin protein in Ae. aegypti with anti‐lachesin antibody resulted in a significant reduction in DENV replication.

中文翻译：

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埃及伊蚊的lachesin蛋白与登革热病毒2的信封蛋白的结构域III结合并抑制病毒复制。

登革热病毒（DENV）由四种血清型（DENV-1至-4）组成，在医学上是最重要的虫媒病毒（节肢动物传播的病毒）之一。DENV感染是人类的主要健康负担，并且通过昆虫媒介埃及伊蚊在人类之间传播。e 埃及y以被感染的人类为食时摄入DENV，在被注入健康人类之前，它会穿过蚊子的肠道，血淋巴和唾液腺。在这种传播过程中，DENV必须与昆虫载体的许多蛋白质相互作用，这对于其成功传播至关重要。我们的研究集中于识别和表征Ae中相互作用的蛋白质伴侣。埃及到DENV。由于包膜蛋白（E）的结构域III（DIII）暴露于病毒体表面并参与病毒进入各种细胞的进入，因此我们针对DENV-2包膜蛋白（EDIII）的结构域III对噬菌体展示文库进行了筛选。发现显示出与lachesin蛋白相似的肽序列与EDIII相互作用。克隆蛋白，异源表达，纯化并用于体外相互作用研究。Lachesin蛋白与EDIII以及DENV相互作用。此外，蜡蛋白蛋白定位在Ae的不同器官的神经元细胞中。伊蚊共聚焦显微镜。在Ae中阻断lachesin蛋白。埃及伊蚊带有抗lachesin抗体导致DENV复制显着减少。