Synthesis and spectroscopic study of dual self‐encoded polymer microbeads with Raman scattering and surface‐enhanced Raman scattering,Journal of Raman Spectroscopy

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Synthesis and spectroscopic study of dual self‐encoded polymer microbeads with Raman scattering and surface‐enhanced Raman scattering
Journal of Raman Spectroscopy ( IF 2.4 ) Pub Date : 2020-03-03 , DOI: 10.1002/jrs.5863
Umar Azhar ₁ , Qazi Ahmed ₁ , Zeyad Alwahabi ₁ , Sheng Dai _{1,

2}

Affiliation

The unique Raman spectra of various polymers render polymeric microbeads to be attractive in Raman‐based multiplex analysis and simultaneous biomolecular recognition as self‐encoded supports. The combination of polymer Raman signals with highly sensitive surface‐enhanced Raman scattering (SERS) codes will be able to significantly enhance the combinatory library in bioanalysis. However, there is lack of study on the preparation and spectroscopic analysis of self‐encoded microbeads with both Raman and SERS signatures. This study establishes a robust synthesis and spectral analysis protocol of the microbeads with bi‐codes, namely Raman and SERS. As modal examples, surface carboxylic acid functionalized monodisperse microbeads of polystyrene and poly(4‐tert‐butylstyrene) were conjugated with the amine group of 4‐aminothiophenol, followed by the adsorption of gold nanoparticles (AuNP) on microbead surfaces. The thiol group of 4‐aminothiophenol forms self‐assembled monolayers with AuNPs. The resulting microbeads are Raman and SERS dual self‐encoded as evident from their Raman spectroscopic and imaging analysis. The synthesis and spectroscopic analysis of the Raman and SERS dual‐encoded systems suggest the potential expansion of multiplex library in suspension bioassays.

中文翻译：

具有拉曼散射和表面增强拉曼散射的双重自编码聚合物微珠的合成和光谱研究

各种聚合物的独特拉曼光谱使聚合物微珠在基于拉曼的多重分析和同时作为自我编码支持物的生物分子识别中具有吸引力。聚合物拉曼信号与高度敏感的表面增强拉曼散射（SERS）码的结合将能够显着增强生物分析中的组合库。但是，对于具有拉曼和SERS签名的自编码微珠的制备和光谱分析尚缺乏研究。这项研究建立了具有双码即Raman和SERS的微珠的鲁棒合成和光谱分析协议。作为模式示例，将聚苯乙烯和聚（4-叔丁基苯乙烯）的表面羧酸官能化单分散微珠与4-氨基硫酚的胺基共轭，然后将金纳米颗粒（AuNP）吸附在微珠表面上。4-氨基苯硫酚的巯基与AuNPs形成自组装单层。从拉曼光谱和成像分析中可以明显看出，产生的微珠是拉曼和SERS双重自编码的。拉曼和SERS双编码系统的合成和光谱分析表明，悬浮生物测定中多重文库的潜在扩展。

更新日期：2020-03-03

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