Ultrasensitive amyloid β‐protein quantification with high dynamic range using a hybrid graphene–gold surface‐enhanced Raman spectroscopy platform,Journal of Raman Spectroscopy

当前位置： X-MOL 学术 › J. Raman Spectrosc. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Ultrasensitive amyloid β‐protein quantification with high dynamic range using a hybrid graphene–gold surface‐enhanced Raman spectroscopy platform
Journal of Raman Spectroscopy ( IF 2.4 ) Pub Date : 2019-12-17 , DOI: 10.1002/jrs.5785
Xinke Yu ₁ , Eric Y Hayden ₂ , Pu Wang ₁ , Ming Xia ₁ , Owen Liang ₁ , Yu Bai ₃ , David B Teplow ₂ , Ya-Hong Xie _{1,

4}

Affiliation

Author(s): Yu, X; Hayden, EY; Wang, P; Xia, M; Liang, O; Bai, Y; Teplow, DB; Xie, YH | Abstract: © 2019 John Wiley a Sons, Ltd. Surface enhanced Raman spectroscopy (SERS) holds great promise in biosensing because of its single-molecule, label-free sensitivity. We describe here the use of a graphene–gold hybrid plasmonic platform that enables quantitative SERS measurement. Quantification is enabled by normalizing analyte peak intensities to that of the graphene G peak. We show that two complementary quantification modes are intrinsic features of the platform and that through their combined use, the platform enables accurate determination of analyte concentration over a concentration range spanning seven orders of magnitude. We demonstrate, using a biologically relevant test analyte, the amyloid β-protein (Aβ), a seminal pathologic agent of Alzheimer's disease, that linear relationships exist between (a) peak intensity and concentration at a single plasmonic hot spot smaller than 100 nm and (b) frequency of hot spots with observable protein signals, that is, the colocation of an Aβ protein and a hot spot. We demonstrate the detection of Aβ at a concentration as low as 10−18 M after a single 20 μl aliquot of the analyte onto the hybrid platform. This detection sensitivity can be improved further through multiple applications of analyte to the platform and by rastering the laser beam with smaller step sizes.

中文翻译：

使用混合石墨烯-金表面增强拉曼光谱平台进行高动态范围的超灵敏β-淀粉样蛋白定量

作者：余X；海登，EY；王，P；夏，M；梁，O；白，Y;泰普洛，DB；谢玉华 |摘要：© 2019 John Wiley a Sons, Ltd. 表面增强拉曼光谱 (SERS) 因其单分子、无标记的灵敏度而在生物传感领域具有巨大的前景。我们在这里描述了使用石墨烯-金混合等离子体平台来实现定量 SERS 测量。通过将分析物峰强度归一化为石墨烯 G 峰强度来实现定量。我们表明，两种互补的定量模式是该平台的固有特征，通过将它们结合使用，该平台能够在跨越七个数量级的浓度范围内准确测定分析物浓度。我们使用生物学相关的测试分析物β淀粉样蛋白（Aβ）（阿尔茨海默病的一种重要病理因子）证明，(a) 小于 100 nm 的单个等离激元热点处的峰强度和浓度之间存在线性关系，（b）具有可观察到的蛋白质信号的热点频率，即Aβ蛋白和热点的共置。我们展示了在混合平台上单份 20 μl 分析物后检测浓度低至 10−18 M 的 Aβ。通过在平台上多次应用分析物以及以更小的步长光栅化激光束，可以进一步提高检测灵敏度。

更新日期：2019-12-17

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11