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Skimmed milk powder and pectin decorated solid lipid nanoparticle containing soluble curcumin used for the treatment of colorectal cancer
Journal of Food Process Engineering ( IF 2.7 ) Pub Date : 2019-10-10 , DOI: 10.1111/jfpe.13246
Muthu M. J. Moideen 1 , Kavitha Karuppaiyan 1 , Ruckmani Kandhasamy 1 , Shanmuganathan Seetharaman 2
Affiliation  

This research is aimed at the preparation of biopolymeric skimmed milk powder (SMP) and pectin coated dual layer solid lipid nanoparticles loaded with soluble curcumin (CMN) to enhance the cytotoxicity of the drug delivered to the colon for colorectal cancer (CRC). The curcumin (C‐) and curcumin solid dispersion (C‐SD) (soluble curcumin) loaded solid lipid nanoparticle (SLN) (C‐SD‐SLN) are prepared by hot homogenization method and characterized its physicochemical properties. The surface of the C‐SD‐SLNs was coated with natural biopolymers of pectin and skimmed milk powder (SMP), called cetyl palmitate (CP)‐SD‐SLN. The resultant nanoparticles showed significantly high drug loading, better stability, and slower release profile (92.1%) in various GI conditions up to 72 H. zebra fish toxicity studies of CP‐SD‐SLN confirm its in vivo safety, and the result from in vitro cytotoxicity showed that 50% inhibition (26 μM/ml) is achieved with an amount of CP‐SD‐SLN, which was considerably lesser than free CMN. The result from flow cytometry analysis confirmed the cell cycle arrest in the G2/M phase (49.11%), and western blot analysis was performed to understand its molecular level apoptosis in Caco‐2 cells. The profound efficiency of SMP and pectin coated CP‐SD‐SLN is a promising preparation that indicated its potential application for CRC treatment without toxicity. PRACTICAL APPLICATION: The pharma and biotech industry is seeking techniques to increase potential and reduce the toxicity of chemotherapeutic agents. The solid lipid nanoparticle (SLN) has fascinated significant attention in anticancer drug delivery generally because of the targeted delivery to the particular site and devoid of other tissues. The present investigation provides the efficiency of skimmed milk powder (SMP)‐coated lipid shell contains soluble curcumin to release the drug at colon and improve the metabolism and therefore increase the potential activity several fold than native curcumin. The results show that SMP and pectin coated SLN is a potential carrier for delivery to the colon and thus improve anticancer potential of human adenocarcinoma (Caco‐2) cells of curcumin. These results will contribute in designing SLN techniques for optimizing the wide‐ranging quality of natural curcumin.

中文翻译:

含可溶性姜黄素的脱脂奶粉和果胶修饰的固体脂质纳米粒用于治疗结直肠癌

本研究旨在制备生物聚合物脱脂奶粉 (SMP) 和负载可溶性姜黄素 (CMN) 的果胶涂层双层固体脂质纳米颗粒,以增强递送至结肠的结肠直肠癌 (CRC) 药物的细胞毒性。姜黄素(C-)和姜黄素固体分散体(C-SD)(可溶性姜黄素)负载固体脂质纳米颗粒(SLN)(C-SD-SLN)是通过热均质法制备的,并表征了其理化性质。C-SD-SLN 的表面涂有果胶和脱脂奶粉 (SMP) 的天然生物聚合物,称为十六烷基棕榈酸酯 (CP)-SD-SLN。所得纳米颗粒在高达 72 H. CP-SD-SLN 的斑马鱼毒性研究证实了其体内安全性,在各种 GI 条件下显示出显着的高载药量、更好的稳定性和更慢的释放曲线 (92.1%),体外细胞毒性的结果表明,使用一定量的 CP-SD-SLN 可实现 50% 的抑制(26 μM/ml),这远低于游离 CMN。流式细胞术分析结果证实细胞周期停滞在 G2/M 期 (49.11%),并进行蛋白质印迹分析以了解其在 Caco-2 细胞中的分子水平凋亡。SMP 和果胶包被的 CP-SD-SLN 的显着效率是一种很有前途的制剂,表明其在无毒性的 CRC 治疗中的潜在应用。实际应用:制药和生物技术行业正在寻求技术来增加化疗药物的潜力并降低其毒性。固体脂质纳米颗粒 (SLN) 在抗癌药物递送中引起了极大的关注,通常是因为它可以靶向递送到特定部位并且没有其他组织。本研究提供了脱脂奶粉 (SMP) 包覆的脂质壳含有可溶性姜黄素的效率,可在结肠释放药物并改善代谢,从而将潜在活性提高数倍于天然姜黄素。结果表明,SMP 和果胶包被的 SLN 是一种潜在的递送至结肠的载体,从而提高姜黄素的人腺癌 (Caco-2) 细胞的抗癌潜力。这些结果将有助于设计用于优化天然姜黄素广泛质量的 SLN 技术。本研究提供了脱脂奶粉 (SMP) 包覆的脂质壳含有可溶性姜黄素的效率,可在结肠释放药物并改善新陈代谢,从而使潜在活性比天然姜黄素增加数倍。结果表明,SMP 和果胶包被的 SLN 是一种潜在的递送至结肠的载体,从而提高姜黄素的人腺癌 (Caco-2) 细胞的抗癌潜力。这些结果将有助于设计用于优化天然姜黄素广泛质量的 SLN 技术。本研究提供了脱脂奶粉 (SMP) 包覆的脂质壳含有可溶性姜黄素的效率,可在结肠释放药物并改善新陈代谢,从而使潜在活性比天然姜黄素增加数倍。结果表明,SMP 和果胶包被的 SLN 是一种潜在的递送至结肠的载体,从而提高姜黄素的人腺癌 (Caco-2) 细胞的抗癌潜力。这些结果将有助于设计用于优化天然姜黄素广泛质量的 SLN 技术。结果表明,SMP 和果胶包被的 SLN 是一种潜在的递送至结肠的载体,从而提高姜黄素的人腺癌 (Caco-2) 细胞的抗癌潜力。这些结果将有助于设计用于优化天然姜黄素广泛质量的 SLN 技术。结果表明,SMP 和果胶包被的 SLN 是一种潜在的递送至结肠的载体,从而提高姜黄素的人腺癌 (Caco-2) 细胞的抗癌潜力。这些结果将有助于设计用于优化天然姜黄素广泛质量的 SLN 技术。
更新日期:2019-10-10
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