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Yunnan Baiyao diminishes lipopolysaccharide-induced inflammation in osteoclasts.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-03-18 , DOI: 10.1111/jfbc.13182 Xiaobin Ren 1 , Yanping Zhu 2 , Liangkun Xie 3 , Mingzhu Zhang 1 , Lihui Gao 4 , Hongbing He 1
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-03-18 , DOI: 10.1111/jfbc.13182 Xiaobin Ren 1 , Yanping Zhu 2 , Liangkun Xie 3 , Mingzhu Zhang 1 , Lihui Gao 4 , Hongbing He 1
Affiliation
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Yunnan Baiyao (YNBY) has been refined for hundreds of years and has become a treasure of proprietary Chinese medicine that has significant curative effects in the field of hemostasis, blood circulation, and callus. In past years, YNBY has been demonstrated to play an anti‐inflammatory role in bone‐related diseases, such as rheumatoid arthritis and osteoporosis. However, the osteoclasts are multinucleated giant cells that resorb bone and participate in the occurrence, development, and progression of these bone‐related diseases. Previous studies have reported that the inflammatory function is closely associated with arachidonic acid (AA) metabolism, as well as some inflammatory‐related pathways, including the nuclear factor кB (NF‐кB), mitogen‐activated protein kinase (MAPK), and Wnt5a pathways. Therefore, we speculated that the anti‐inflammatory effect of YNBY might be associated with the NF‐кB, MAPK, and Wnt5a pathways. In order to further excavate the anti‐inflammatory roles of YNBY, lipopolysaccharide (LPS) with an optimal concentration of 1,000 pg/ml was used to induce inflammation in osteoclasts. Our results showed that YNBY with a time‐ and dose‐dependent method decreased the concentration of pro‐inflammatory cytokines and the expression levels of cyclooxygenase‐1 (COX‐1), COX‐2, 5‐lipoxygenase, and prostaglandin E2. Moreover, it was found that COX‐2 was the target gene regulated by YNBY. Finally, using NF‐кB and MAPK pathway inhibitors or miRNA101b (involved in the Wnt5a pathway) in tandem with YNBY and the results exhibited that these groups caused a reduction in COX‐1 and COX‐2 expression, indicating that the anti‐inflammatory function of YNBY might directly affect the NF‐кB, MAPK, and Wnt5a pathways.
中文翻译：
云南白药减轻了破骨细胞中脂多糖引起的炎症。
云南白药（YNBY）经过数百年的提炼，已成为中成药的瑰宝，在止血，血液循环和愈伤组织等领域具有重要的治疗作用。在过去的几年中，YNBY被证明在类风湿性关节炎和骨质疏松症等骨相关疾病中具有抗炎作用。然而，破骨细胞是多核巨细胞，可吸收骨并参与这些骨相关疾病的发生，发展和发展。先前的研究报道炎症功能与花生四烯酸（AA）代谢以及某些与炎症相关的途径密切相关，包括核因子кB（NF-кB），促分裂原激活蛋白激酶（MAPK）和Wnt5a途径。因此，我们推测YNBY的抗炎作用可能与NF-кB，MAPK和Wnt5a途径有关。为了进一步发挥YNBY的抗炎作用，最适浓度为1,000 pg / ml的脂多糖（LPS）用于诱导破骨细胞的炎症。我们的结果表明，YNBY采用时间和剂量依赖性方法可以降低促炎细胞因子的浓度以及环氧合酶-1（COX-1），COX-2、5-脂氧合酶和前列腺素E2的表达水平。此外，还发现COX-2是YNBY调控的靶基因。最后，将NF‐кB和MAPK途径抑制剂或miRNA101b（参与Wnt5a途径）与YNBY一起使用，结果表明这些基团导致COX-1和COX-2表达降低，
更新日期：2020-03-18
中文翻译：
云南白药减轻了破骨细胞中脂多糖引起的炎症。
云南白药（YNBY）经过数百年的提炼，已成为中成药的瑰宝，在止血，血液循环和愈伤组织等领域具有重要的治疗作用。在过去的几年中，YNBY被证明在类风湿性关节炎和骨质疏松症等骨相关疾病中具有抗炎作用。然而，破骨细胞是多核巨细胞，可吸收骨并参与这些骨相关疾病的发生，发展和发展。先前的研究报道炎症功能与花生四烯酸（AA）代谢以及某些与炎症相关的途径密切相关，包括核因子кB（NF-кB），促分裂原激活蛋白激酶（MAPK）和Wnt5a途径。因此，我们推测YNBY的抗炎作用可能与NF-кB，MAPK和Wnt5a途径有关。为了进一步发挥YNBY的抗炎作用，最适浓度为1,000 pg / ml的脂多糖（LPS）用于诱导破骨细胞的炎症。我们的结果表明，YNBY采用时间和剂量依赖性方法可以降低促炎细胞因子的浓度以及环氧合酶-1（COX-1），COX-2、5-脂氧合酶和前列腺素E2的表达水平。此外，还发现COX-2是YNBY调控的靶基因。最后，将NF‐кB和MAPK途径抑制剂或miRNA101b（参与Wnt5a途径）与YNBY一起使用，结果表明这些基团导致COX-1和COX-2表达降低，
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