Tannin fraction of pistachio green hull extract with pancreatic lipase inhibitory and antioxidant activity.,Journal of Food Biochemistry

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Tannin fraction of pistachio green hull extract with pancreatic lipase inhibitory and antioxidant activity.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-03-18 , DOI: 10.1111/jfbc.13208
Zohre Noorolahi ₁ , Mohammad Ali Sahari ₁ , Mohsen Barzegar ₁ , Hassan Ahmadi Gavlighi ₁

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Polyphenolic compounds (present in different parts of the plant) have beneficial properties such as antioxidant and inhibition of key enzymes. In this research, antioxidant and anti‐lipase activity of pistachio green hull (PGH) extract was investigated. Fractionation of PGH on Sephadex LH‐20 furnished a tannin enriched fraction with higher antioxidant activity respect to that of the extract and of the non‐tannin fraction. UHPL/MS² analyses showed the presence of phenolic compounds including galloyl‐O‐hexoside, galloyl‐shikimic acid, galloylquinic acid, and gallic acid in tannin fraction. PGH‐extract was an un‐competitive inhibitor against porcine pancreatic lipase so that its IC₅₀ value was 2.26 mg/ml. In the same phenol amount (490 µg), anti‐lipase activity of the tannin fraction was also more than non‐tannin fraction and crude PGH‐extract. This is probably due to the presence of some active polyphenolic compounds such as gallic acid.

中文翻译：

阿月浑子绿色壳提取物的单宁含量具有胰腺脂肪酶抑制和抗氧化活性。

多酚化合物（存在于植物的不同部位）具有有益的特性，例如抗氧化剂和关键酶的抑制作用。在这项研究中，对开心果绿壳（PGH）提取物的抗氧化和抗脂肪酶活性进行了研究。在Sephadex LH-20上分馏PGH可得到富含单宁的馏分，其抗氧化活性高于提取物和非单宁的馏分。UHPL / MS ^2个分析表明酚类化合物包括galloyl-的存在Ó -hexoside，没食子酰基莽草酸，galloylquinic酸，和单宁分数没食子酸。PGH提取物是抗猪胰脂肪酶的非竞争性抑制剂，因此其IC ₅₀值为2.26mg / ml。在相同的苯酚量（490微克）下，单宁级分的抗脂肪酶活性也要高于非单宁级分和粗制的PGH提取物。这可能是由于某些活性多酚化合物（例如没食子酸）的存在。

更新日期：2020-03-18

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