Microcystin‐leucine arginine (MC‐LR) is a cyclic heptapeptide hepatotoxin produced by cyanobacteria. MicroRNA‐122 (miR‐122) is specifically expressed in the liver. This study focuses on the role of miR‐122 in MC‐LR‐induced dysregulation of hepatic iron homeostasis in C57BL/6 mice. The thirty mice were randomly divided into five groups (Control, 12.5 μg/kg·BW MC‐LR, 25 μg/kg·BW MC‐LR, Negative control agomir and 25 μg/kg·BW MC‐LR + miR‐122 agomir). The results show that MC‐LR decreases the expressions of miR‐122, Hamp, and its related regulators, while increasing the content of hepatic iron and the expressions of FPN1 and Tmprss6. Furthermore, miR‐122 agomir pretreatment improves MC‐LR induced dysregulation of hepatic iron homeostasis by arousing the related regulators and reducing the expression of Tmprss6. These results suggest that miR‐122 agomir can prevent the accumulation of hepatic iron induced by MC‐LR, which may be related to the regulation of hepcidin by BMP/SMAD and IL‐6/STAT signaling pathways.

中文翻译：

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microRNA-122在微囊藻毒素-亮氨酸精氨酸诱导的小鼠肝脏铁稳态失调中的作用

微囊藻毒素-亮氨酸精氨酸 (MC-LR) 是一种由蓝藻产生的环状七肽肝毒素。MicroRNA-122 (miR-122) 在肝脏中特异性表达。本研究的重点是 miR-122 在 MC-LR 诱导的 C57BL/6 小鼠肝脏铁稳态失调中的作用。将三十只小鼠随机分为五组（Control, 12.5 μg/kg·BW MC-LR, 25 μg/kg·BW MC-LR, Negative control agomir and 25 μg/kg·BW MC-LR + miR-122 agomir ）。结果表明，MC-LR降低了miR-122、Hamp及其相关调节因子的表达，同时增加了肝铁含量和FPN1和Tmprss6的表达。此外，miR-122 agomir预处理通过激发相关调节因子和降低Tmprss6的表达来改善MC-LR诱导的肝脏铁稳态失调。