The primary metabolize of chlorpyrifos (CPF) is in the liver tissue, which it can cause oxidative damage and apoptosis in liver cells. The use of exercise with antioxidant supplements could have a protective effects in the liver tissue especially by improve mitochondria function. The aim of the present study was to investigate the protective effect of aerobic exercise and eugenol (Eu) supplementation on destructive effects of CPF in liver tissue. Sixty‐four adult male albino rats were randomly divided into eight groups (eight rats in each group). Four experimental groups received intraperitoneal injection of either 3.0 mg/kg body weight CPF in dimethyl sulfoxide for six consecutive weeks. Aerobic exercise was performed 5 days per week over 4 weeks for exercise groups. Finally, the animals were sacrificed for the histomorphometric analysis and biochemical measurement in the liver tissue. The result of this study show that consumption of CPF alone, caused collagen deposition, increased apoptosis, tumor necrosis factor α, malondialdehyde, and decreased catalase, superoxide dismutase, acetylcholinesterase (AChE) compared to control and exercise groups (healthy groups) in liver tissue (P ˂ .05). Prescription of exercises and Eu supplements in CPF consumer groups, neutralized this destructive effects of CPF. However, concomitant administration of Eu with exercise had better effects on liver tissue (P ˂ .05). It seems that consumption of Eu with aerobic exercise have a protective role in tissue destruction, inflammatory damage by improving antioxidant defense and modulating AChE activity in hepatocytes.

中文翻译：

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有氧运动和丁香酚补充剂通过调节乙酰胆碱酯酶激活和抗氧化防御改善毒死蜱诱导的肝损伤

毒死蜱（CPF）的主要代谢产物在肝组织中，可引起肝细胞氧化损伤和凋亡。使用抗氧化剂补充剂进行运动可以对肝脏组织产生保护作用，尤其是通过改善线粒体功能。本研究的目的是研究有氧运动和丁香酚 (Eu) 补充剂对 CPF 对肝组织破坏作用的保护作用。64只成年雄性白化大鼠随机分为8组（每组8只）。四个实验组连续六周接受腹膜内注射 3.0 mg/kg 体重 CPF 的二甲亚砜溶液。运动组在 4 周内每周进行 5 天的有氧运动。最后，将动物处死用于肝组织中的组织形态计量学分析和生化测量。这项研究的结果表明，与对照组和运动组（健康组）相比，单独食用 CPF 会导致肝组织中的胶原沉积、细胞凋亡、肿瘤坏死因子 α、丙二醛和过氧化氢酶、超氧化物歧化酶、乙酰胆碱酯酶 (AChE) 降低(P ˂ .05)。CPF 消费群体中的运动处方和 Eu 补充剂，抵消了 CPF 的这种破坏性影响。然而，与运动同时服用 Eu 对肝组织有更好的影响（P ˂ .05）。通过有氧运动消耗 Eu 似乎通过改善抗氧化防御和调节肝细胞中的 AChE 活性在组织破坏、炎症损伤中具有保护作用。