Cardamonin, a monomeric alkaloid, is isolated from Alpinia conchigera Griff and other natural plants. Recently, it has been focused on its anticancer activities, and no information showing its immune effects on leukemia mice was reported. In this study, we investigated the immune effects of cardamonin on WEHI‐3 cell–generated leukemia mice. Forty BALB/c mice were randomly divided into four groups: Group I mice were normal animals and groups II‐IV were leukemia. Group II mice, as a positive control, were administered with normal diet, and group III and IV mice were treated with 1 and 5 mg/kg of cardamonin, respectively, by intraperitoneal injection every 2 days for 14 days. The population of white blood cells, macrophage phagocytosis, and the proliferations of T and B cells were analyzed by flow cytometry. Another forty mice were also separated randomly into four groups for the determination of survival rate. Results showed that cardamonin did not affect body weight. Cardamonin decreased CD3, CD11b, and Mac‐3 cell populations but increased CD19 number. Cardamonin enhanced phagocytic abilities of macrophages from the peripheral blood mononuclear cells of leukemia mice. Furthermore, cardamonin at 1 mg/kg treatment improved the survival rate of leukemia mice in vivo. Therefore, cardamonin could be applied for a leukemia therapeutic reagent at a defined dose.

中文翻译：

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小豆蔻素在体内诱导免疫反应并提高 WEHI-3 细胞产生的小鼠白血病的存活率

小豆蔻素是一种单体生物碱，是从 Alpinia conchigera Griff 和其他天然植物中分离出来的。最近，它一直专注于其抗癌活性，但没有报道显示其对白血病小鼠的免疫作用。在这项研究中，我们研究了小豆蔻素对 WEHI-3 细胞产生的白血病小鼠的免疫作用。40只BALB/c小鼠随机分为四组：I组小鼠为正常动物，II-IV组小鼠为白血病。II组小鼠作为阳性对照，正常饮食，III组和IV组小鼠分别用1和5mg/kg小豆蔻素腹腔注射，每2天一次，共14天。通过流式细胞术分析白细胞群、巨噬细胞吞噬作用以及T和B细胞的增殖。另外40只小鼠也随机分成四组用于测定存活率。结果表明，小豆蔻素不影响体重。豆蔻素减少了 CD3、CD11b 和 Mac-3 细胞群，但增加了 CD19 数量。豆蔻素增强了白血病小鼠外周血单核细胞巨噬细胞的吞噬能力。此外，1 mg/kg 的小豆蔻素治疗提高了体内白血病小鼠的存活率。因此，小豆蔻素可用于确定剂量的白血病治疗试剂。豆蔻素增强了白血病小鼠外周血单核细胞巨噬细胞的吞噬能力。此外，1 mg/kg 的小豆蔻素治疗提高了体内白血病小鼠的存活率。因此，小豆蔻素可用于确定剂量的白血病治疗试剂。豆蔻素增强了白血病小鼠外周血单核细胞巨噬细胞的吞噬能力。此外，1 mg/kg 的小豆蔻素治疗提高了体内白血病小鼠的存活率。因此，小豆蔻素可用于确定剂量的白血病治疗试剂。