Biosurfactants Production Using Permeate from Whey Ultrafiltration and Bioproduct Recovery by Membrane Separation Process,Journal of Surfactants and Detergents

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Biosurfactants Production Using Permeate from Whey Ultrafiltration and Bioproduct Recovery by Membrane Separation Process
Journal of Surfactants and Detergents ( IF 1.6 ) Pub Date : 2020-02-19 , DOI: 10.1002/jsde.12399
Andressa Decesaro ₁ , Thaís Strieder Machado ₁ , Ângela Carolina Cappellaro ₂ , Alan Rempel ₁ , Ana Cláudia Margarites ₃ , Christian Oliveira Reinehr ₄ , Marcos Nogueira Eberlin _{5,

6} , Davila Zampieri _{5,

6,

7} , Antônio Thomé ₁ , Luciane Maria Colla ₁

Affiliation

The management of whey is a challenge for dairy products where the volume produced is remarkable. This problem is minimized through membrane separation processes (MSP) to obtain whey protein concentrate, which has high added value. However, a permeate effluent stream is still generated that is composed of lactose, vitamins, and minerals, which can serve as raw material for the production of biotechnological compounds. Thus, this study aimed to produce biosurfactants using the permeate from whey ultrafiltration as part of the culture media of the bioprocess, to recover the biosurfactant produced using MSP, and to identify the biocompound. The production was carried out using Bacillus methylotrophicus and Bacillus pumilus. The variables nitrogen source (urea or ammonium sulfate), nitrogen source concentration (0.5% or 1.0%), inducer (soybean oil or biodiesel), inducer concentration (1% or 2%), and the addition of micronutrients (with our without) were studied using a fractional factorial experimental design 2^5‐1_IV. In the fermentation processes, it was possible to verify the biosurfactant production through the reduction of surface tension, obtaining a minimum value of 35.07 mN/m for B. methylotrophicus and 26.02 mN/m for B. pumilus. Recovery via MSP was an efficient strategy for biosurfactant purification, which was concentrated in the fraction of the retentate. We produced a high‐value‐added biocompound identified as surfactin, valuing the permeate residue from whey ultrafiltration.

中文翻译：

使用乳清超滤渗透物生产生物表面活性剂和通过膜分离工艺回收生物产品

对于乳制品而言，乳清的管理是一项挑战，因为乳制品的产量非常可观。通过膜分离工艺（MSP）可以使该问题最小化，从而获得具有高附加值的乳清蛋白浓缩物。但是，仍会产生由乳糖，维生素和矿物质组成的渗透物流出物流，它们可以用作生产生物技术化合物的原料。因此，本研究旨在使用乳清超滤中的渗透物作为生物过程培养基的一部分来生产生物表面活性剂，以回收利用MSP生产的生物表面活性剂并鉴定生物化合物。使用甲基芽孢杆菌和短小芽孢杆菌进行生产。变量氮源（尿素或硫酸铵），氮源浓度（0.5％或1.0％），诱导剂（大豆油或生物柴油），诱导剂浓度（1％或2％）以及微量营养素的添加（无添加）使用分数阶乘实验设计2 ^5-1_{IV进行研究}。在发酵过程中，可以通过降低表面张力来验证生物表面活性剂的产生，对于甲基营养芽孢杆菌而言，最小值为35.07 mN / m，对于短小芽孢杆菌而言，最小值为26.02 mN / m 。通过恢复MSP是生物表面活性剂纯化的有效策略，它浓缩在截留物中。我们生产了鉴定为表面活性素的高附加值生物化合物，对乳清超滤过程中的渗透残留物进行了评估。

更新日期：2020-02-19

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