Determination of the distribution of sophorolipid (SL) congeners controls their self‐assembly structures and therefore their employment as glycolipid biosurfactants in cosmetics, environmental remediation, and personal care applications. The compatibility of 3‐hydroxypicolinic acid (HPA) as a matrix for compositional analysis of SL using palm olein as carbon energy source by matrix‐assisted laser desorption ionization time of flight mass spectrometry (MALDI‐TOF MS) analysis under positive and negative ion modes is studied in this work. Examination in positive ion mode revealed ions that were not detected in negative ion mode. Molecular ions produced using HPA matrix were lactonic Glc‐Glc‐1′,4″‐C18:1, anionic Glc‐Glc‐1′‐C18:1, lactonic monoacetate of Glc‐Glc‐1′,4″‐C18:1, and anionic monoacetate Glc‐Glc‐1′‐C18:1 and the anionic diacetate of Glc‐Glc‐1′‐C18:1. We also detected Glc‐Glc‐1′‐C18:0, GlcOAc‐Glc‐1′‐C18:0, GlcOAc‐GlcOAc‐1′,4″‐C18:0, and GlcOAc—GlcOAc‐1′‐C18:0 which are congeners of SL with stearic acid moieties. 3‐HPA has been established to be the superior matrix for elucidation of structures for SL using MALDI‐TOF MS.

中文翻译：

---

3-羟基吡啶甲酸作为基质辅助激光解吸电离飞行时间质谱用于槐糖脂结构解析的有效基质

确定槐糖脂（SL）同类物的分布可控制其自组装结构，因此可控制其在化妆品，环境修复和个人护理应用中作为糖脂生物表面活性剂的使用。3-羟基吡啶甲酸（HPA）作为基质的相容性，用于在正负离子模式下通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析使用棕榈油精作为碳源的SL的成分分析在这项工作中被研究。在正离子模式下检查发现在负离子模式下未检测到离子。使用HPA基质产生的分子离子为内酯Glc-Glc-1'，4” -C18：1，阴离子Glc-Glc-1'-C18：1，内酯Glc-Glc-1'，4” -C18：1 ，以及阴离子单乙酸酯Glc-Glc-1'-C18：1和Glc-Glc-1'-C18：1的阴离子二乙酸盐。我们还检测到Glc‐Glc‐1′‐C18：0，GlcOAc‐Glc‐1′‐C18：0，GlcOAc‐GlcOAc‐1′，4″ ‐C18：0和GlcOAc-GlcOAc‐1′‐C18：0是具有硬脂酸部分的SL的同类物。3-HPA已被确立为使用MALDI-TOF MS阐明SL结构的优良基质。