ABSTRACT

Herein, we report the characterization of chitosan-based cues incorporated with two different essential oils, i.e., tea tree oil (TTO) and clove oil (CO). The essential oils incorporated chitosan-based cues offer considerable solutions to eliminate pathogenic infections and/or to reduce microbial contaminations in healthcare facilities. In this background, chitosan(CST)-based cues were incorporated with TTO and CO and final cues were designated as CST-TTO and CST-CO. The loading efficiency was optimal in CST-TTO (91.5%) followed by CST-CO (83.3%). The optimally yielded both, CST-TTO and CST-CO were tested for antibacterial activities against two Gram+ve (Staphylococcus aureus, and Bacillus subtilis) and two Gram-ve strains (Escherichia coliandSalmonella typhimurium). An initial bacterial cell count (1.5 x 10⁸ CFU/mL) was considered as a control value, and 2-log reduction was considered to report the antibacterial activity. The antifungal activity was tested against two Aspergillus strains, i.e., Aspergillus niger and Aspergillus flavus. The growth reduction for Aspergillus strains was recorded from 1 × 10⁸ conidia/mL (initial count) to 1 × 10² conidia/mL (optimal inhibition) in the presence of CST-TTO and CST-CO. In summary, the results suggest that the newly developed CST-TTO and CST-CO cues can be potential candidates for food and biomedical applications as they hold promising capability to restrict some pathogenic and food spoilage microbes.

摘要

在本文中，我们报告了结合有两种不同的精油即茶树油（TTO）和丁香油（CO）的壳聚糖基线索的特征。掺有脱乙酰壳多糖的线索的精油提供了可观的解决方案，以消除病原体感染和/或减少医疗机构中的微生物污染。在这种背景下，将基于壳聚糖（CST）的线索与TTO和CO结合在一起，最终线索被指定为CST-TTO和CST-CO。CST-TTO（91.5％），CST-CO（83.3％）的装载效率最佳。测试了最佳产率的CST-TTO和CST-CO对两种革兰氏阳性菌（金黄色葡萄球菌和枯草芽孢杆菌）和两种革兰氏菌株（大肠杆菌和大肠杆菌）的抗菌活性。鼠伤寒沙门氏菌）。初始细菌细胞计数（1.5 x 10 ⁸ CFU / mL）被视为对照值，并且2-log降低被认为报告了抗菌活性。测试了针对两种曲霉菌株，即黑曲霉和黄曲霉的抗真菌活性。记录的曲霉菌株的生长降低从1×10 ⁸分生孢子/ mL（初始计数）到1×10 ²分生孢子/ mL（最佳抑制）在存在CST-TTO和CST-CO的情况下。总之，结果表明，新开发的CST-TTO和CST-CO线索具有潜在的限制某些病原性和食物变质微生物的能力，因此它们可能是食品和生物医学应用的潜在候选者。