ABSTRACT

Present work aimed to examine antioxidant, anti-inflammatory and anti-mutagenic effects of senna and fennel via synergetic mechanisms against deleterious effects of gamma radiation exposure. Cytogenetic, biochemical and immunological investigations were evaluated in human blood lymphocyte cultures. Samples were divided into Group I: control, Group II: irradiated samples with 3Gy γ-irradiation, Group III: treated samples with a mixture of calcium sennosides and fennel seed extract and Group IV: irradiated treated samples. The results revealed that radiation exposure caused an increment in numbers of micronuclei (MN), nucleoplasmic bridges (NPBs), mono-, and multinucleated cells. Biochemical results revealed a decrease in levels of SOD and CAT enzymes and an increase in LDH level after irradiation. Levels of cytokines (IL-1β, IL-6, IL-8, and TNF- α) were significantly increased in irradiated group. Calcium sennosides and fennel seed extract mixture treatment after radiation exposure succeeded in returning cytokines and antioxidant enzyme levels to their normal levels as compared to control. Also, group IV showed a marvelous improvement in numbers of MN and NPB frequencies. These results indicate that the combination between Calcium sennosides as free radicals scavenger and fennel as an apoptic inducer can be used as a post-radiation treatment against oxidative and inflammation effects of ionizing radiation.

摘要

目前的工作旨在通过协同作用机制来检查番泻叶和茴香的抗氧化，抗炎和抗诱变作用，以对抗γ射线辐射的有害作用。在人类血液淋巴细胞培养物中评估了细胞遗传学，生化和免疫学研究。将样品分为I组：对照，II组：经3Gyγ辐射的辐照样品，III组：含钙番石榴苷和茴香籽提取物混合物的处理过的样品，IV组：经辐照的处理过的样品。结果表明，辐射暴露导致微核（MN），核质桥（NPB），单核和多核细胞数量增加。生化结果显示，辐照后SOD和CAT酶水平降低，LDH水平升高。细胞因子水平（IL-1β，IL-6，IL-8，辐照组TNF-α和TNF-α）明显升高。与对照相比，放射线照射后的钙番石榴苷和茴香籽提取物混合物处理成功地使细胞因子和抗氧化酶水平恢复至正常水平。同样，第四组显示了MN和NPB频率数量的惊人改善。这些结果表明，钙仙奴糖苷作为自由基清除剂和茴香作为细胞凋亡诱导剂的组合可以用作放射后治疗，以对抗电离辐射的氧化和炎症作用。第四组显示MN和NPB频率的惊人改善。这些结果表明，钙仙奴糖苷作为自由基清除剂和茴香作为凋亡诱导剂的组合可以用作放射后治疗，以对抗电离辐射的氧化和炎症作用。第四组显示MN和NPB频率的惊人改善。这些结果表明，钙仙奴糖苷作为自由基清除剂和茴香作为细胞凋亡诱导剂的组合可以用作放射后治疗，以对抗电离辐射的氧化和炎症作用。