ABSTRACT

Foeniculum vulgare Mill. (commonly known as fennel) is one of the precious spices. Almost all parts of the plant are edible and have powerful interest in phytochemical and pharmacological research. The present study was designed to examine the free radical scavenging potential, oxidative damage preventive activity, and anti–inflammatory effects of traditionally used fennel. It assessed the cytogenetic and biochemical effects of fennel on suppression of the radiation hazards in human blood culture at a working dose of 200µg/ml. The treatment time was 72 h post-irradiation (3 Gy γ-irradiation). Triple blood cultures for each blood sample were set up. Ionizing radiation exposure induced significant increase in micronuclei (MN) frequencies in both mono- and binucleated cells beside significant decrease in superoxide dismutase (SOD) and catalase (CAT) activities and increase in lactate dehydrogenase (LDH). While Interleukin (IL)-1β, −6, −8 and Tumor Necrosis Factor (TNF)-α levels were significantly increased after irradiation. Treatment with fennel exhibited a decrease in micronuclei and enhancement in the level of SOD, CAT, and LDH activities. In addition, significant amelioration in IL-1β, 6 and 8, TNF-α activity was scored. The present results revealed the antimutagenic, anti–inflammatory and antioxidant effects of fennel against oxidative stress induced by γ- irradiation.

摘要

普通茴香磨。（俗称茴香）是珍贵的香料之一。植物的几乎所有部分都可以食用，并且对植物化学和药理学研究具有浓厚的兴趣。本研究旨在检查清除自由基的潜力，预防氧化损伤的活性以及传统茴香的抗炎作用。该研究评估了在200μg/ ml的工作剂量下茴香对抑制人类血液培养中的辐射危害的细胞遗传学和生化作用。处理时间为照射后72小时（3 Gyγ照射）。建立每个血液样品的三重血液培养物。电离辐射暴露引起单核细胞和双核细胞中微核（MN）频率的显着增加，同时超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性显着降低，乳酸脱氢酶（LDH）升高。照射后白介素（IL）-1β，-6，-8和肿瘤坏死因子（TNF）-α水平显着升高。茴香处理显示微核减少，SOD，CAT和LDH活性增加。另外，对IL-1β，6和8的明显改善进行了评分。目前的结果表明，茴香具有抗诱变，抗炎和抗氧化作用，可抵抗γ-射线诱导的氧化应激。照射后-8和肿瘤坏死因子（TNF）-α水平显着增加。茴香处理显示微核减少，SOD，CAT和LDH活性增加。另外，对IL-1β，6和8的明显改善进行了评分。目前的结果表明，茴香具有抗诱变，抗炎和抗氧化作用，可抵抗γ-射线诱导的氧化应激。照射后-8和肿瘤坏死因子（TNF）-α水平显着增加。茴香处理显示微核减少，SOD，CAT和LDH活性增加。另外，对IL-1β，6和8的明显改善进行了评分。目前的结果表明，茴香具有抗诱变，抗炎和抗氧化作用，可抵抗γ-射线诱导的氧化应激。