ABSTRACT A new warm-water benthic dinoflagellate species, Coolia guanchica sp. nov., is described from the Canary Islands (NE Atlantic Ocean), a subtropical archipelago with a rich benthic biodiversity. Samples of benthic microalgae were collected at Tenerife, and three strains of a previously unreported ribotype of the genus Coolia were found. Identification was carried out by combining morphological (light microscope, LM; and scanning electron microscope, SEM) and molecular (large subunit ribosomal gene, LSU rRNA; and internal transcribed spacer region, ITS1-5.8S-ITS2 rRNA) data from clonal cultures. Phylogenetic analyses revealed that the new strains formed a well-supported clade, sister to Coolia canariensis. The genetic distance of more than 0.20 for LSU and 0.30 for the ITS region (uncorrected p values) from the closest members of the genus (C. canariensis and C. cf. canariensis) showed species-level divergence to justify the description of a new species, Coolia guanchica sp. nov. A bioassay with Artemia salina was used to check the toxicity of the species, with negative results. Coolia guanchica has rounded cells, with plate 4′ as the largest plate of the epitheca and plate 6′′ about twice as wide as long, similar to C. canariensis and C. areolata. It differs morphologically from the other two ribotypes in the C. canariensis complex in having a lighter hypothecal ornamentation, larger thecal plate pores scattered randomly throughout the cell and higher pore density per thecal plate.

中文翻译：

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Coolia guanchica sp. 十一月 （甲藻科）一种来自加那利群岛（大西洋东北部）的新底栖甲藻

摘要 一种新的温水底栖甲藻，Coolia guanchica sp。11 月，来自加那利群岛（大西洋东北部），这是一个亚热带群岛，底栖生物多样性丰富。在特内里费岛收集了底栖微藻样本，并发现了三种先前未报告的苦力属核糖型菌株。通过结合来自克隆培养物的形态学（光学显微镜，LM；和扫描电子显微镜，SEM）和分子（大亚基核糖体基因，LSU rRNA；和内部转录间隔区，ITS1-5.8S-ITS2 rRNA）数据进行鉴定。系统发育分析表明，新菌株形成了一个支持良好的进化枝，是 Coolia canariensis 的姐妹。LSU 的遗传距离大于 0.20，而 0. 来自该属最接近成员（C. canariensis 和 C. cf. canariensis）的 ITS 区域（未校正的 p 值）的 30 显示了物种水平的差异，以证明对新物种 Coolia guanchica sp. 的描述是合理的。十一月 使用卤虫的生物测定来检查该物种的毒性，结果为阴性。Coolia guanchica 细胞呈圆形，4' 板是上皮的最大板，6'' 板的宽度约为 C. canariensis 和 C. areolata 的两倍。它在形态学上与 C. canariensis 复合体中的其他两种核糖型的不同之处在于具有更轻的假设纹饰，更大的膜板孔随机散布在整个细胞中，每个膜板的孔密度更高。Coolia guanchica sp. 十一月 使用卤虫的生物测定来检查该物种的毒性，结果为阴性。Coolia guanchica 细胞呈圆形，4' 板是上鞘最大的板，6'' 板的宽度大约是其长的两倍，类似于 C. canariensis 和 C. areolata。它在形态学上与 C. canariensis 复合体中的其他两种核糖型的不同之处在于具有更轻的假设纹饰，更大的膜板孔随机散布在整个细胞中，每个膜板的孔密度更高。Coolia guanchica sp. 十一月 使用卤虫的生物测定来检查该物种的毒性，结果为阴性。Coolia guanchica 细胞呈圆形，4' 板是上皮的最大板，6'' 板的宽度约为 C. canariensis 和 C. areolata 的两倍。它在形态学上与 C. canariensis 复合体中的其他两种核糖型的不同之处在于具有更轻的假设纹饰，更大的膜板孔随机散布在整个细胞中，每个膜板的孔密度更高。