ABSTRACT The present study was designed to isolate petroleum hydrocarbon degrading bacterial strains from crude oil contaminated sites near the ARAMCO (Jazan, Saudi Arabia). The main aim was to identify a novel microbial comminutes that possess high degradation potential under variant environmental conditions. Total 43 crude oil degrading bacterial consortiums were isolated and six strains were selected based on high growth rate and hydrocarbon degradation ability. The results of 16S rRNA gene sequences analysis showed that these isolated strains were belonged to genus Pseudomonas, Nitratireductor, Bacillus, Staphylococcus and Erwinia. All isolates bacterial strains showed high degradation potential, however, strain 2-IV (Pseudomonas stutzeri) was the most effective for all parameters. The strain 2-IV showed high degradation rate (77%), emulsifying activity (54.5%), and hydrophobicity (65.5%). Moreover, the production of bio-surfactants and great decrease in surface tension (30.5 mN m−1) was also observed for strain 2-IV compared to the other isolates. On the basis of current research it was concluded that these bacterial strain 2-II and 2-IV can be used for cleaning the environment, respectively. However, further study is needed for identification of fungi and actinomycetes that can act on long-chain hydrocarbons in the studied area.

中文翻译：

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中温细菌降解来自沙特阿拉伯阿美公司不同地点的原油的特征

摘要 本研究旨在从阿美石油公司（沙特阿拉伯吉赞）附近的原油污染地点分离石油烃降解细菌菌株。主要目的是确定一种在不同环境条件下具有高降解潜力的新型微生物粉碎物。共分离出43株原油降解菌群，根据生长速度快、烃类降解能力强，筛选出6株。16S rRNA基因序列分析结果表明，这些分离菌株属于假单胞菌属、硝化还原菌属、芽孢杆菌属、葡萄球菌属和欧文氏菌属。所有分离菌株都显示出高降解潜力，然而，菌株 2-IV（施氏假单胞菌）对所有参数最有效。菌株 2-IV 显示出高降解率 (77%)，乳化活性 (54.5%) 和疏水性 (65.5%)。此外，与其他分离株相比，菌株 2-IV 还观察到生物表面活性剂的产生和表面张力的大幅降低 (30.5 mN m-1)。根据目前的研究得出结论，这些菌株 2-II 和 2-IV 可分别用于清洁环境。然而，需要进一步研究以鉴定可作用于研究区域的长链碳氢化合物的真菌和放线菌。分别。然而，需要进一步研究以鉴定可作用于研究区域的长链碳氢化合物的真菌和放线菌。分别。然而，需要进一步研究以鉴定可作用于研究区域的长链碳氢化合物的真菌和放线菌。