Abstract It is well-known that the high-molecular-weight polycyclic aromatic hydrocarbons (HMW-PAHs) are difficult to degrade, and they can cause tremendous damaging effects in environment. In this article, a novel dibenz[a,h]anthracene (DBA)-degrading strain Amycolatopsis sp. Y1-2 was isolated and identified from the contaminated soils in the coal mining areas. The degradation of HMW-PAHs by strain Y1-2 could be improved by adopting DBA, benzo(a)anthracene, benzo(b)fluoranthene, benzo(a)pyrene, and benzo(g,hi)perylene as the sole carbon and energy sources. The co-metabolism substrates test revealed that the HMW-PAHs degradation efficiency of strain Y1-2 could be significantly improved under co-metabolism substrates, especially glucose (increased by 34.70%) and cyclodextrin. The optimization results of DBA-degrading conditions obtained from response surface analysis are as follows: pH 7.6, concentration of DBA 10.71 mg L−1, inoculum amount 1.58 g 100 mL−1 medium, concentration of glucose 9.98 g L−1, and concentration of cyclodextrin 2.05 g L−1. The predicted value of degradation percentage in the optimized conditions was 99.03% (very similar with the actual value of 97.84%). Based on the above results, the strain Y1-2 can degrade HMW-PAHs effectively and will be applied in treating PAHs-containing soils and industrial wastewater.

中文翻译：

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Dibenz[a,h] 蒽降解菌株 Amycolatopsis sp。来自煤矿区土壤的 Y1-2

摘要 众所周知，高分子量多环芳烃（HMW-PAHs）难降解，对环境造成巨大破坏。在本文中，一种新的二苯并 [a,h] 蒽 (DBA) 降解菌株 Amycolatopsis sp。Y1-2是从煤矿区污染土壤中分离鉴定出来的。采用DBA、苯并(a)蒽、苯并(b)荧蒽、苯并(a)芘和苯并(g,hi)苝作为唯一碳和能量可以改善菌株Y1-2对HMW-PAHs的降解来源。共代谢底物试验表明，在共代谢底物，尤其是葡萄糖（增加34.70%）和环糊精的作用下，菌株Y1-2的HMW-PAHs降解效率得到显着提高。响应面分析得到的DBA降解条件优化结果如下：pH 7.6，DBA浓度10.71 mg L-1，接种量1.58 g 100 mL-1培养基，葡萄糖浓度9.98 g L-1，浓度环糊精 2.05 g L-1。优化条件下降解百分比的预测值为99.03%（与实际值97.84%非常相似）。综合以上结果，菌株Y1-2能有效降解HMW-PAHs，可用于处理含PAHs的土壤和工业废水。03%（与实际值 97.84% 非常相似）。综合以上结果，菌株Y1-2能有效降解HMW-PAHs，可用于处理含PAHs的土壤和工业废水。03%（与实际值 97.84% 非常相似）。综合以上结果，菌株Y1-2能有效降解HMW-PAHs，可用于处理含PAHs的土壤和工业废水。