Galactomannans are a widely used polysaccharide. In this study, galactomannans were oxidized using galactose oxidase, aided by horseradish peroxidase (HRP) and catalase. Then, a small amount of hydrogen peroxide was used to oxidize the reaction products in order to prepare the paper strengthening agents. The optimal oxidation conditions were investigated according to the properties of the paper, which included the galactose oxidase dosages and concentrations of hydrogen peroxide. It was determined that, when the enzyme dosage was 50 U/g and the hydrogen peroxide concentration was 0.4%, the properties of the paper could be evidently improved with a lower hydrogen peroxide addition. When 1.5% oxidized galactomannans were used, the tensile index and folding endurance were found to have been increased by 23.50% and 110.31%, respectively, when compared with the control. In regard to the recycled paper, the increases were observed to have reached 22.19% and 175%, respectively. The results of the molecular weight, FT-IR, and CP/MAS13C-NMR indicated that a percentage aldehyde and carboxyl groups had been added to the galactomannans.

中文翻译：

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半乳甘露聚糖的半乳糖氧化酶空气氧化用作纸张增强剂

半乳甘露聚糖是一种广泛使用的多糖。在这项研究中，在辣根过氧化物酶 (HRP) 和过氧化氢酶的辅助下，使用半乳糖氧化酶氧化半乳甘露聚糖。然后，使用少量过氧化氢氧化反应产物以制备纸张增强剂。根据纸张的性质研究了最佳氧化条件，包括半乳糖氧化酶剂量和过氧化氢浓度。经测定，当酶用量为50 U/g，双氧水浓度为0.4%时，过氧化氢添加量较低，纸张性能有明显改善。当使用 1.5% 氧化半乳甘露聚糖时，拉伸指数和耐折度分别提高了 23.50% 和 110.31%，与对照相比。至于再生纸，据观察，增幅分别达到 22.19% 和 175%。分子量、FT-IR 和 CP/MAS13C-NMR 的结果表明半乳甘露聚糖中加入了一定比例的醛和羧基。