Abstract Succinic acid has been characterized as a commodity chemical and attractive compound as it has wide applications as a key building block for various high value-added chemicals. In this study, simultaneous saccharification and fermentation (SSF) process conditions for the production of succinic acid from oil palm empty fruit bunches by Actinobacillus succinogenes (A. succinogenes) were statistically optimized for the first time. Factors that influence succinic acid production, such as temperature, pH, and cellulase loading, were screened using full factorial design. The experimental conditions of SSF for succinic acid production were optimized by varying the temperature (36 °C, 38 °C, and 40 °C), pH (5, 6.5, and 8), and cellulase loading (10, 25, and 40 FPU/g). The results revealed that these three factors had significant effects on succinic acid production. Response surface methodology (RSM) was used to optimize the operating conditions of SSF to obtain a high yield of succinic acid. The optimum conditions for SSF occurred at 36 °C, pH 5, and cellulase loading of 39.7 FPU/g, with succinic acid production of 42.9 g/L and a conversion yield of 0.61 g/g. The validation results showed that the experimental values of succinic acid were in agreement to RSM prediction with a small percentage error.

中文翻译：

---

油棕空果串同步糖化发酵生产琥珀酸工艺条件优化

摘要 琥珀酸被认为是一种商品化学品和有吸引力的化合物，因为它作为各种高附加值化学品的关键组成部分具有广泛的应用。本研究首次对产琥珀酸放线杆菌（A. succinogenes）从油棕空果串生产琥珀酸的同步糖化发酵（SSF）工艺条件进行了统计优化。使用全因子设计筛选影响琥珀酸生产的因素，例如温度、pH 值和纤维素酶加载量。通过改变温度（36 °C、38 °C 和 40 °C）、pH（5、6.5 和 8）和纤维素酶负载（10、25 和 40 FPU/克）。结果表明，这三个因素对琥珀酸的产量有显着影响。响应面法 (RSM) 用于优化 SSF 的操作条件以获得高产率的琥珀酸。SSF 的最佳条件发生在 36 °C、pH 5 和纤维素酶负载量为 39.7 FPU/g，琥珀酸产量为 42.9 g/L，转化率为 0.61 g/g。验证结果表明，琥珀酸的实验值与RSM预测一致，误差较小。