ABSTRACT Curcuma mangga is a medicinal plant, and its rhizomes are often used to treat various conditions, such as fever, thorax pain, itching, stomachaches, skin diseases, gout, and asthma. Although C. mangga is commonly used, information on the relationship between its chemical constituents and the bioactivities of the rhizomes is still limited. The extraction solvents used have a strong effect on the metabolite profile and the bioactivity of the extract. A nuclear magnetic resonance (NMR)-based metabolomics approach was used to differentiate the metabolite profiles of hexane, chloroform, ethyl acetate, and methanol fractions of C. mangga rhizomes and to correlate the metabolites with α-glucosidase inhibitory activity. Primary and secondary metabolites were identified, including curcuminoids, carbohydrates, terpenoids, and amino acids. The ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) analysis of the most active fraction (ethyl acetate) revealed the identification of additional metabolites, such as zerumin A, epigallocatechin, p-hydroxycinnamic, and copallic acids. A partial least square (PLS) biplot demonstrated that the existence of curcumin, demethoxycurcumin, curcumanggoside, calcaratarin A, labda-8(17),12-diene-15,16-dial, zerumin B, and difurocumenonol in the ethyl acetate fraction could be responsible for the α-glucosidase inhibitory activity.

中文翻译：

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从姜黄组分中鉴定α-葡萄糖苷酶抑制化合物

摘要 姜黄是一种药用植物，其根茎常用于治疗各种病症，如发热、胸痛、瘙痒、胃痛、皮肤病、痛风和哮喘。尽管 C. mangga 是常用的，但关于其化学成分与根茎生物活性之间关系的信息仍然有限。所使用的提取溶剂对提取物的代谢物谱和生物活性有很大影响。使用基于核磁共振 (NMR) 的代谢组学方法来区分 C. mangga 根状茎的己烷、氯仿、乙酸乙酯和甲醇部分的代谢物谱，并将代谢物与 α-葡萄糖苷酶抑制活性相关联。鉴定了初级和次级代谢物，包括类姜黄素、碳水化合物、萜类化合物和氨基酸。对活性最强的部分（乙酸乙酯）进行超高效液相色谱-串联质谱 (UPLC-MS/MS) 分析，揭示了其他代谢物的鉴定结果，例如 Zerumin A、表没食子儿茶素、对羟基肉桂酸和柯帕酸。偏最小二乘 (PLS) 双标图表明，乙酸乙酯馏分中姜黄素、去甲氧基姜黄素、姜黄苷、calcaratarin A、labda-8(17)、12-diene-15,16-dial、zerumin B 和 difurocumenonol 的存在可以负责α-葡萄糖苷酶抑制活性。