ABSTRACT The objective of this study was to investigate the influence of granule-bound starch-binding domain fusion gene expression on the structure and properties of potato starch. The glgB/GASBD fusion gene and the glgB gene for control were transferred into the potato cultivar Desiree using Agrobacterium-mediated method, which were expressed in potato tubers driven by the potato granule-bound starch synthase gene promoter. The accumulation of glgB/GASBD fusion protein in starch granules was analyzed by Western blot hybridization. A large amount of glgB/GASBD fusion protein was found to accumulate in the starch granules, and the position of the GASBD in the fusion protein (at the N-terminus or at the C-terminus) had no significant effect on the accumulation of the fusion protein in the starch granules. An increase in the degree of branching of the starch results in a specific change in the physical and chemical properties and functional properties of the starch.

中文翻译：

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glgB/GASBD融合基因表达对马铃薯淀粉分枝度增加及淀粉理化性质变化的影响

摘要 本研究的目的是研究颗粒结合淀粉结合域融合基因表达对马铃薯淀粉结构和特性的影响。使用农杆菌介导的方法将 glgB/GASBD 融合基因和用于控制的 glgB 基因转移到马铃薯品种 Desiree 中，它们在马铃薯颗粒结合淀粉合酶基因启动子的驱动下在马铃薯块茎中表达。通过蛋白质印迹杂交分析glgB/GASBD融合蛋白在淀粉颗粒中的积累。发现大量glgB/GASBD融合蛋白在淀粉颗粒中积累，GASBD在融合蛋白中的位置（N端或C端）对glgB/GASBD的积累没有显着影响。淀粉颗粒中的融合蛋白。