ABSTRACT

Chitosan-microparticles (CM)-coating (CMC) effect on quality and shelf-life of vacuum-packed sea bream (Sparus aurata) fillets was investigated at 0.2% and0.5% (w/w) doses comparatively to untreated control, during refrigerated storage (24 days). Analysis concerned total mesophilic bacteria counts (TMC), total psychrophilic bacteria counts (TPC), proximate composition, pH, total volatile basic nitrogen (TVB-N), trimethylamine (TMA), thiobarbyturic acid (TBA), and fatty acids (FA). Two-way ANOVA and multiple comparison were applied, factors being storage time and treatment. Bacterial counts exceeded the limit in control but remained below in CMC lots. All of TVB-N, TMA and TBA increased faster and reached higher values in control compared to CMC lots, with dose-dependent effect. CMC preserved quality in terms of proximate composition and FA. The 0.5%-CM dose prolonged the shelf-life up to 12 days, corresponding to a two folds extension. CMC showed antimicrobial and antioxidant properties and should be recommended for preserving the quality of fish fillets.

摘要

壳聚糖微粒（CM）涂层（CMC）对真空包装鲷鱼（Sparus aurata）的质量和保质期的影响在冷藏期间（24天），与未处理的对照组相比，以0.2％和0.5％（w / w）的剂量研究了鱼片。分析涉及中温细菌总数（TMC），嗜冷细菌总数（TPC），附近成分，pH，总挥发性碱性氮（TVB-N），三甲胺（TMA），硫代巴比妥酸（TBA）和脂肪酸（FA） 。应用了两次方差分析和多重比较，因素是储存时间和处理。细菌数量超过了对照的限制，但仍低于CMC批次。与CMC批次相比，所有TVB-N，TMA和TBA的增长速度更快，并且在对照中达到更高的值，且具有剂量依赖性。CMC在邻近的成分和FA方面保持质量。0.5％-CM剂量可将保质期延长至12天，相当于延长了两倍。